The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver
| العنوان: | The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver |
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| المؤلفون: | Grégory Chevillard, Valérie Nicolas-Francès, Stéphane Mandard, F. Hansmannel, Frank J. Gonzalez, J. A. Bonzo, Thierry Pineau, Stephen A. Duncan, Michele A. Battle, Pascal G.P. Martin, J. Chamouton, Norbert Latruffe, Marie Claude Clémencet |
| المساهمون: | Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Epidémiologie des maladies chroniques : impact des interactions gène environnement sur la santé des populations, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute [Bethesda] (NCI-NIH), National Institutes of Health [Bethesda] (NIH)-National Institutes of Health [Bethesda] (NIH), Lady Davis Institute for Medical Research [Montréal], McGill University = Université McGill [Montréal, Canada]-Jewish General Hospital, Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Laboratoire de Pharmacologie et Toxicologie, UR66, INRA, Institut National de la Recherche Agronomique (INRA), The Ministry of Research, National Education and Technology, the Regional Council of Burgundy, the GDR-CNRS no2583 on peroxisome, the French Ministry of Research, National Education and Technology., McGill University-Jewish General Hospital, Mandard, Stéphane, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Lille, Droit et Santé, National Cancer Institute ( NIH ), Lady Davis Institute for Medical Research, McGill University, Institut National de la Recherche Agronomique ( INRA ) |
| المصدر: | PPAR Research PPAR Research, Hindawi Publishing Corporation, 2010, 2010, pp.352957. ⟨10.1155/2010/352957⟩ PPAR Research (2010), 352957. (2010) PPAR Research, Hindawi Publishing Corporation, 2010, 2010, pp.352957. 〈10.1155/2010/352957〉 PPAR Research, Vol 2010 (2010) |
| بيانات النشر: | Hindawi Publishing Corporation, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Article Subject, Response element, Peroxisome proliferator-activated receptor, Biology, 03 medical and health sciences, Transactivation, 0302 clinical medicine, Drug Discovery, Gene expression, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, SDV:BBM, Pharmacology (medical), [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, lcsh:QH301-705.5, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Transcription factor, 030304 developmental biology, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, chemistry.chemical_classification, Genetics, Endocrinology and metabolism, 0303 health sciences, Thiolase, Intron, [ SDV.MHEP.EM ] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Cell biology, lcsh:Biology (General), Nuclear receptor, chemistry, 030220 oncology & carcinogenesis, Endocrinologie et métabolisme, Research Article |
| الوصف: | PPARα and HNF4α are nuclear receptors that control gene transcription by direct binding to specific nucleotide sequences. Using transgenic mice deficient for either PPARα or HNF4α, we show that the expression of the peroxisomal3-keto-acyl-CoA thiolase B(Thb) is under the dependence of these two transcription factors. Transactivation and gel shift experiments identified a novel PPAR response element within intron 3 of theThbgene, by which PPARα but not HNF4α transactivates. Intriguingly, we found that HNF4α enhanced PPARα/RXRα transactivation from TB PPRE3 in a DNA-binding independent manner. Coimmunoprecipitation assays supported the hypothesis that HNF4α was physically interacting with RXRα. RT-PCR performed with RNA from liver-specific HNF4α-null mice confirmed the involvement of HNF4α in the PPARα-regulated induction ofThbby Wy14,643. Overall, we conclude that HNF4α enhances the PPARα-mediated activation ofThbgene expression in part through interaction with the obligate PPARα partner, RXRα. |
| وصف الملف: | text/xhtml; application/pdf |
| اللغة: | English |
| تدمد: | 1687-4757 1687-4765 |
| DOI: | 10.1155/2010/352957 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2005d876aff6d3d8a8af08249fdcb1f6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2005d876aff6d3d8a8af08249fdcb1f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16874757 16874765 |
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| DOI: | 10.1155/2010/352957 |