Hypoxia-induced lactate dehydrogenase A protects cells from apoptosis in endometriosis
| العنوان: | Hypoxia-induced lactate dehydrogenase A protects cells from apoptosis in endometriosis |
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| المؤلفون: | Na Liu, Qianmeng Huang, Jinyan Zheng, Li-Bing Shi, Xiang Lin, Y Dai, Feng Zhou, Songying Zhang, Xiaoying Jin |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | endometriosis, Adult, Cancer Research, Stromal cell, Epithelial-Mesenchymal Transition, Lactate dehydrogenase A, Cell, lactate dehydrogenase A, Apoptosis, Protective Agents, Biochemistry, Flow cytometry, Endometrium, Genetics, medicine, Humans, Glycolysis, Lactic Acid, RNA, Messenger, education, Molecular Biology, hypoxia-inducible factor 1α, Cell Proliferation, education.field_of_study, Oncogene, medicine.diagnostic_test, L-Lactate Dehydrogenase, Chemistry, hypoxia, Epithelial Cells, Articles, Cell cycle, glycolysis, Molecular biology, medicine.anatomical_structure, Oncology, Molecular Medicine, Female, Lactate Dehydrogenase 5, Stromal Cells, Reactive Oxygen Species |
| الوصف: | The pathological expression and function of lactate dehydrogenase A (LDHA), a key enzyme that converts pyruvate into lactic acid during glycolysis, remains unknown in endometriosis. In the present study, LDHA expression in tissue samples was determined by immunohistochemistry. To examine whether LDHA was induced by hypoxia, primary cultured endometrial stromal cells (ESCs) and glandular epithelial Ishikawa cells were exposed to 1% O2 (hypoxia) or 21% O2 (normoxia). Cellular functions were assessed by flow cytometry, Transwell and Cell Counting Kit‑8 assays in LDHA‑silenced ESCs and Ishikawa cells. Mitochondrial functions were evaluated using mitochondrial membrane potential JC‑1 staining, reactive oxygen species flow cytometric analysis and ATP detection. Additionally, lactic acid production was examined and western blotting was used to evaluate the expression levels of proteins associated with apoptosis, cell cycle and glycolysis, as well as regulatory proteins involved in epithelial‑mesenchymal transformation and glycolytic pathways. LDHA was localized to endometrial glandular cells and stromal cells. However, LDHA protein expression was higher in endometriotic lesions compared with that in normal and eutopic endometria. LDHA expression levels in ectopic glandular cells were higher during the proliferative stage compared with during the secretory stage. Hypoxia treatment of Ishikawa cells and ESCs markedly induced the mRNA and protein expression of LDHA. Silencing of LDHA expression in Ishikawa cells and THESC cells significantly promoted impaired mitochondrial function and apoptosis while inhibiting migration and glycolysis. However, it had no obvious effect on proliferation. In conclusion, the present study revealed that LDHA was highly expressed in endometriotic tissues, where it may serve a notable role in the occurrence and development of endometriosis. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1eebafe01efe25d5a931c91725b41178Test http://europepmc.org/articles/PMC8281285Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1eebafe01efe25d5a931c91725b41178 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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