Differential hepatoprotective mechanisms of rutin and quercetin in CCl4-intoxicated BALB/cN mice
| العنوان: | Differential hepatoprotective mechanisms of rutin and quercetin in CCl4-intoxicated BALB/cN mice |
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| المؤلفون: | Vanja Vasiljev Marchesi, Sanda Vladimir-Knežević, Robert Domitrović, Dario Rahelić, Žarko Tadić, Hrvoje Jakovac, Željko Romić, Olga Cvijanović |
| المصدر: | Acta Pharmacologica Sinica. 33:1260-1270 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Rutin, medicine.medical_treatment, Intraperitoneal injection, Pharmacology, Nitric Oxide, medicine.disease_cause, Nitric oxide, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Liver Function Tests, Picrates, medicine, Animals, heterocyclic compounds, Pharmacology (medical), 030304 developmental biology, Mice, Inbred BALB C, 0303 health sciences, Dose-Response Relationship, Drug, Molecular Structure, biology, Carbon Tetrachloride Poisoning, Chemistry, Nitrotyrosine, Biphenyl Compounds, Free Radical Scavengers, General Medicine, Immunohistochemistry, 3. Good health, Nitric oxide synthase, Biphenyl compound, Oxidative Stress, Liver, Biochemistry, 030220 oncology & carcinogenesis, biology.protein, Quercetin, Original Article, Chemical and Drug Induced Liver Injury, Injections, Intraperitoneal, Oxidative stress |
| الوصف: | To investigate the mechanisms underlying the protective effects of quercetin-rutinoside (rutin) and its aglycone quercetin against CCl(4)-induced liver damage in mice.BALB/cN mice were intraperitoneally administered rutin (10, 50, and 150 mg/kg) or quercetin (50 mg/kg) once daily for 5 consecutive days, followed by the intraperitoneal injection of CCl(4) in olive oil (2 mL/kg, 10% v/v). The animals were sacrificed 24 h later. Blood was collected for measuring the activities of ALT and AST, and the liver was excised for assessing Cu/Zn superoxide dismutase (SOD) activity, GSH and protein concentrations and also for immunoblotting. Portions of the livers were used for histology and immunohistochemistry.Pretreatment with rutin and, to a lesser extent, with quercetin significantly reduced the activity of plasma transaminases and improved the histological signs of acute liver damage in CCl(4)-intoxicated mice. Quercetin prevented the decrease in Cu/Zn SOD activity in CCl(4)-intoxicated mice more potently than rutin. However, it was less effective in the suppression of nitrotyrosine formation. Quercetin and, to a lesser extent, rutin attenuated the inflammation in the liver by down-regulating the CCl(4)-induced activation of nuclear factor-kappa B (NF-κB), tumor necrosis factor-α (TNF-α) and cyclooxygenase (COX-2). The expression of inducible nitric oxide synthase (iNOS) was more potently suppressed by rutin than by quercetin. Treatment with both flavonoids significantly increased NF-E2-related factor 2 (Nrf2) and heme oxygenase (HO-1) expression in injured livers, although quercetin was less effective than rutin at an equivalent dose. Quercetin more potently suppressed the expression of transforming growth factor-β1 (TGF-β1) than rutin.Rutin exerts stronger protection against nitrosative stress and hepatocellular damage but has weaker antioxidant and anti-inflammatory activities and antifibrotic potential than quercetin, which may be attributed to the presence of a rutinoside moiety in position 3 of the C ring. |
| تدمد: | 1745-7254 1671-4083 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ee50187a1dd5bf5d5d23b2e73dc324dTest https://doi.org/10.1038/aps.2012.62Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1ee50187a1dd5bf5d5d23b2e73dc324d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17457254 16714083 |
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