Upregulation of microRNA‑300 induces the proliferation of liver cancer by downregulating transcription factor FOXO1
| العنوان: | Upregulation of microRNA‑300 induces the proliferation of liver cancer by downregulating transcription factor FOXO1 |
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| المؤلفون: | Guangbing Wei, Xuqi Li, Yuanhong Chang, Xinming Chang, Cancan Zhou, Guanglin Qiu, Lin Fan, Guanghui Wang |
| المصدر: | Oncology Reports. |
| بيانات النشر: | Spandidos Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Cancer Research, Carcinoma, Hepatocellular, FOXO1, P70-S6 Kinase 1, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, medicine, Animals, Humans, Luciferase, 3' Untranslated Regions, Protein kinase B, Transcription factor, Cell Proliferation, Oncogene, Forkhead Box Protein O1, Chemistry, Liver Neoplasms, Cancer, Hep G2 Cells, General Medicine, Middle Aged, medicine.disease, Up-Regulation, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer research, Female, Liver cancer, Neoplasm Transplantation, Signal Transduction |
| الوصف: | In the present study, we investigated whether miRNA‑300 (miR‑300) is an oncogene in human liver cancer and sought to determine the mechanism underlying its activity. We also investigated the effect of miRNA‑300 on the growth in liver cancer. To identify its target molecule, we performed luciferase assays. The downstream signaling pathway was detected by immunohistochemical (IHC) analysis in human HCC tissues, and the protein levels of AKT, 4E‑BP1, S6K1, SNAIL and MMP2 were determined using western blotting. miR‑300 levels were higher in patients with high‑stage HCC, and miR‑300 promoted cell growth both in vitro and in vivo. miRNA‑300 inhibited the luciferase activity of FOXO1 by targeting its 3'‑untranslated region (UTR), and overexpression of miR‑300 upregulated the protein levels of phospho‑AKT, phospho‑4E‑BP1, phospho‑S6K1, SNAIL and MMP2. These data revealed that miRNA‑300 functions as an oncogene in liver cancer by inhibiting FOXO1 and interacting with the AKT/mTOR signaling pathway. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e6460d883f6168e2ef63e884f766ec8Test https://doi.org/10.3892/or.2018.6727Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1e6460d883f6168e2ef63e884f766ec8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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