MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1
| العنوان: | MicroRNA-582-5p suppresses non-small cell lung cancer cells growth and invasion via downregulating NOTCH1 |
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| المؤلفون: | Jiaoyu Rao, Xiaokang Wang, Jianghong Liu, Kaiyuan Huang, Xiaoyan Deng, Gengrui Xu, Shengshuo Liu |
| المصدر: | PLoS ONE, Vol 14, Iss 6, p e0217652 (2019) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Lung Neoplasms, Biochemistry, Lung and Intrathoracic Tumors, 0302 clinical medicine, Carcinoma, Non-Small-Cell Lung, Medicine and Health Sciences, Receptor, Notch1, 3' Untranslated Regions, Gene knockdown, Multidisciplinary, Middle Aged, Enzymes, Up-Regulation, Nucleic acids, Gene Expression Regulation, Neoplastic, Oncology, Cell Processes, 030220 oncology & carcinogenesis, Hyperexpression Techniques, Medicine, Female, Oxidoreductases, Luciferase, Research Article, Science, Down-Regulation, Biology, Research and Analysis Methods, Cell Growth, 03 medical and health sciences, Cell Line, Tumor, microRNA, Gene Expression and Vector Techniques, Genetics, medicine, Vimentin, Humans, Gene silencing, Neoplasm Invasiveness, Gene Silencing, Molecular Biology Techniques, Non-coding RNA, Lung cancer, Molecular Biology, Cell Proliferation, Molecular Biology Assays and Analysis Techniques, Natural antisense transcripts, Base Sequence, Cell growth, Cancers and Neoplasms, Biology and Life Sciences, Proteins, Cancer, Cell Biology, medicine.disease, Non-Small Cell Lung Cancer, Gene regulation, Cytoskeletal Proteins, MicroRNAs, 030104 developmental biology, Cell culture, Enzymology, Cancer research, RNA, Ectopic expression, Gene expression |
| الوصف: | Non-small cell lung cancer (NSCLC) is the most common cancer worldwide. MicroRNAs have been shown to be correlated with biological processes of various tumors. In this study, we observed that the expression of miR-582-5p was lower in NSCLC tissues than that in para-carcinoma tissues. Ectopic expression of miR-582-5p significantly inhibited NCI-H358 cell proliferation and invasion. Knockdown of miR-582-5p showed the opposite results, with cell growth rate and the invasive capacity of PC-9 cells enhanced. Furthermore, we elucidated that NOTCH1 is a target of miR-582-5p and there is an inverse correlation between miR-582-5p and NOTCH1 expression in NSCLC tissues. Overexpression of NOTCH1 in miR-582-5p-overexpressing NCI-H358 cells could partially reverse the inhibition of cell proliferation and invasion by miR-582-5p. Thus, our research demonstrated that miR-582-5p suppresses NSCLC cell lines' growth and invasion via targeting oncoprotein NOTCH1 and restoration of miR-582-5p might be feasible therapeutic strategy for NSCLC. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e1af6be10f7fe50bc4fc31acb29a7e1Test https://doi.org/10.1371/journal.pone.0217652Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1e1af6be10f7fe50bc4fc31acb29a7e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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