Analysis of Antiapoptosis Effect of Netrin-1 on Ischemic Stroke and Its Molecular Mechanism under Deleted in Colon Cancer/Extracellular Signal-Regulated Kinase Signaling Pathway
| العنوان: | Analysis of Antiapoptosis Effect of Netrin-1 on Ischemic Stroke and Its Molecular Mechanism under Deleted in Colon Cancer/Extracellular Signal-Regulated Kinase Signaling Pathway |
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| المؤلفون: | Liangqun Rong, Kai Wang, Qingxiu Zhang, Xiue Wei |
| المصدر: | BioMed Research International, Vol 2020 (2020) BioMed Research International |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Brain Infarction, medicine.medical_specialty, Article Subject, MAP Kinase Signaling System, Apoptosis, 030204 cardiovascular system & hematology, General Biochemistry, Genetics and Molecular Biology, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Netrin, medicine, Extracellular, Animals, cardiovascular diseases, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Ischemic Stroke, General Immunology and Microbiology, Behavior, Animal, Kinase, business.industry, Cerebral infarction, fungi, Brain, General Medicine, Netrin-1, medicine.disease, DCC Receptor, Magnetic Resonance Imaging, nervous system diseases, Endocrinology, nervous system, cardiovascular system, Medicine, Signal transduction, business, 030217 neurology & neurosurgery, Immunostaining, Research Article |
| الوصف: | To analyze the regulatory effect of Netrin-1 in ischemic stroke and its influence on Deleted in Colon Cancer (DCC)/Extracellular Signal-regulated Kinase (ERK) signaling pathway, 20 male rats were selected to construct the rat model of middle cerebral artery occlusion (MCAO), 10 normal rats were selected as healthy controls (Normal Saline (NS)), and they were divided into the MCAO+Netrin-1 group, MCAO group, and NS group according to different treatment schemes. The positive expression of Netrin-1 was detected by immunostaining, magnetic resonance imaging (MRI) was adopted to detect the percentage of rat cerebral infarct volume in the cerebral hemispheres, and Modified Neurological Severity Score (mNSS) was adopted to evaluate postoperative neurological function in rats. Besides, a tunnel staining experiment was applied to detect the apoptosis rate of rat neurons, the sticker removal test was applied to evaluate the postoperative sensory function of rats, and fluorescence staining was adopted to detect the expression of DCC and ERK in rats. The results showed that the percentage of cerebral infarction volume in the cerebral hemispheres of the MCAO+Netrin-1 group was higher than that of the MCAO and NS groups ( P < 0.05 ); in the MCAO+Netrin-1 group, the MCAO mNSS scoring and the time spent in the sticker removal test were lower than the MCAO group ( P < 0.05 ); the apoptosis rate of rats in the MCAO+Netrin-1 group was lower than that in the MCAO group ( P < 0.05 ); the average fluorescence intensity of DCC and p-ERK in the MCAO+Netrin-1 group was higher than that in the MCAO group ( P < 0.05 ); the average fluorescence intensity of p-ERK in the MCAO+Netrin-1 group was higher than that in the MCAO group ( P < 0.05 ). In short, Netrin-1 can effectively reduce the brain tissue damage in rats with ischemic stroke, improve the nerve function and sensory function of rats, and inhibit neuronal cell apoptosis. Netrin-1 can promote DCC expression and ERK phosphorylation, and the EPK signaling pathway may be involved in the antiapoptotic effect of Netrin-1. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bd7dccf8b2978f538977d3728e3eda4Test https://pubmed.ncbi.nlm.nih.gov/33274229Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1bd7dccf8b2978f538977d3728e3eda4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 |
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