Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose
| العنوان: | Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose |
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| المؤلفون: | H. G. Eibs, H. Spielmann, C. Mentzel |
| المصدر: | Experientia. 32:1085-1086 |
| بيانات النشر: | Springer Science and Business Media LLC, 1976. |
| سنة النشر: | 1976 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Biology, Chromatography, Affinity, Sepharose, Matrix (chemical analysis), Mice, Cellular and Molecular Neuroscience, Affinity chromatography, Internal medicine, Testis, medicine, Animals, Molecular Biology, Pharmacology, chemistry.chemical_classification, Chromatography, L-Lactate Dehydrogenase, Ldh isozymes, Elution, Cell Biology, NAD, In vitro, Isoenzymes, Endocrinology, Enzyme, chemistry, Molecular Medicine, Lactate dehydrogenase-X |
| الوصف: | At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. In a second step LDH-X is bound to the same matrix at lower NADH and NaCl concentrations and the pure enzyme can subsequently be eluted.Results of a method of rapid purification of lactate dehydrogenase X (LDH-X) from mouse testes by 2 stepts of affinity chromatography on oxamate-sepharose at different sodium chloride (NaCl) and NADH concentrations are discussed. At concentrations of 200 mcM NADH and .5 M NaCl, LDH-X is separated from other LDH isoenzymes. The binding of LDH-X to the column is then carried out on the unbound fractions containing LDH-X and contaminating proteins after a 1:5 dilution. The resulting NADH and NaCl concentrations allow the binding of LDH-X. The specific activity of purified mouse LDH-X from testes was 120 IU/mg. The main advantage of this technique is the rapidity and ease of purification. |
| تدمد: | 1420-9071 0014-4754 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bd0900df58f3cbc0d719c36091b2c8bTest https://doi.org/10.1007/bf01933984Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1bd0900df58f3cbc0d719c36091b2c8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14209071 00144754 |
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