Application of microfluidic technology to the BIOMED-2 protocol for detection of B-cell clonality
| العنوان: | Application of microfluidic technology to the BIOMED-2 protocol for detection of B-cell clonality |
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| المؤلفون: | Marco Chilosi, Francesca Mancini, Annemiek W.M. van Raaij, Alberto Zamò, Marina Montresor, Anna Bertolaso, Aldo Scarpa, Maria Scardoni, Johan H. J. M. van Krieken, Fabio Menestrina, Patricia J. T. A. Groenen |
| المصدر: | Journal of Molecular Diagnostics, 14, 30-7 Journal of Molecular Diagnostics, 14, 1, pp. 30-7 |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Translational research Renal disorder [ONCOL 3], Lymphoma, B-Cell, BIOMED-2 protocol, lymphoproliferative disorders, PCR, GeneScan, clonal peaks, Immunoglobulins, Biology, Sensitivity and Specificity, Pathology and Forensic Medicine, Electrophoresis, Microchip, Capillary electrophoresis, medicine, Humans, Gene, B cell, Gene Rearrangement, B-Lymphocytes, Gene rearrangement, Translational research Tissue engineering and pathology [ONCOL 3], Molecular biology, medicine.anatomical_structure, Polyclonal antibodies, Heavy chain gene, biology.protein, Molecular Medicine, Chain gene, Lymph Nodes, Immunoglobulin Heavy Chains, Heteroduplex |
| الوصف: | Contains fulltext : 109691.pdf (Publisher’s version ) (Closed access) The BIOMED-2 protocol is widely used for detecting clonality in lymphoproliferative disorders. The protocol requires multiple PCR reactions, which are analyzed by either capillary electrophoresis (GeneScan analysis) or heteroduplex PAGE analysis. We tested a microfluidic chip-based electrophoresis device (Agilent 2100 Bioanalyzer) for the analysis of B-cell clonality using PCR for the three framework subregions (FR) of the Ig heavy chain gene (IGH) and PCR for two rearrangements occurring in the Ig kappa chain gene (IGK-VJ and IGK-DE). We analyzed 62 B-cell lymphomas (33 follicular and 29 nonfollicular) and 16 reactive lymph nodes. Chip-based electrophoresis was conclusive for monoclonality in 59/62 samples; for 20 samples, it was compared with GeneScan analysis. Concordant results were obtained in 45/55 IGH (FR1, FR2, and FR3) gene rearrangements, and in 34/37 IGK gene rearrangements. However, when the chip device was used to analyze selected IGK gene rearrangements (biallelic IGK rearrangements or IGK rearrangements in a polyclonal background), its performance was not completely accurate. We conclude, therefore, that this microfluidic chip-based electrophoresis device is reliable for testing cases with dominant PCR products but is less sensitive than GeneScan in detecting clonal peaks in a polyclonal background for IGH PCR, or with complex IGK rearrangement patterns. 01 januari 2012 |
| وصف الملف: | application/pdf |
| تدمد: | 1525-1578 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b1d064559dcea05dd20759962ba76abTest https://doi.org/10.1016/j.jmoldx.2011.07.007Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1b1d064559dcea05dd20759962ba76ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15251578 |
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