Protective effects of growth hormone-releasing hormone analogs in DSS-induced colitis in mice
| العنوان: | Protective effects of growth hormone-releasing hormone analogs in DSS-induced colitis in mice |
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| المؤلفون: | Andrew V. Schally, Annalisa Chiavaroli, Riccarda Granata, Sheila Leone, Claudio Ferrante, Wei Sha, Serena Veschi, Giustino Orlando, Renzhi Cai, Valentina Di Valerio, Iacopo Gesmundo, Lucia Recinella, Rossano Lattanzio, Luigi Brunetti |
| المصدر: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lipopolysaccharides, medicine.medical_specialty, Molecular biology, Science, Biopsy, medicine.medical_treatment, Anti-Inflammatory Agents, Prostaglandin, Inflammation, Growth Hormone-Releasing Hormone, Protective Agents, Dinoprostone, Article, Mice, chemistry.chemical_compound, In vivo, Internal medicine, Animals, Medicine, Intestinal Mucosa, Colitis, Nitrites, Multidisciplinary, L-Lactate Dehydrogenase, business.industry, Growth factor, Dextran Sulfate, Gastroenterology, Growth hormone–releasing hormone, medicine.disease, digestive system diseases, Disease Models, Animal, Endocrinology, chemistry, Cytokines, Tumor necrosis factor alpha, Inflammation Mediators, medicine.symptom, business, Biomarkers, Hormone |
| الوصف: | Besides its metabolic and endocrine effects, growth hormone (GH)-releasing hormone (GHRH) is involved in the modulation of inflammation. Recently synthetized GHRH antagonist MIA-690 and MR-409, GHRH agonist, developed by us have shown potent pharmacological effects in various experimental paradigms. However, whether their administration modify resistance to chronic inflammatory stimuli in colon is still unknown. Ex vivo results demonstrated that MIA-690 and MR-409 inhibited production of pro-inflammatory and oxidative markers induced by lipopolysaccharide on isolated mouse colon specimens. In vivo, both MIA-690 and MR-409 have also been able to decrease the responsiveness to nociceptive stimulus, in hot plate test. Additionally, both peptides also induced a decreased sensitivity to acute and persistent inflammatory stimuli in male mice, in formalin test and dextran sodium sulfate (DSS)-induced colitis model, respectively. MIA-690 and MR-409 attenuate DSS-induced colitis with particular regard to clinical manifestations, histopathological damage and release of pro-inflammatory and oxidative markers in colon specimens. Respect to MR-409, MIA-690 showed higher efficacy in inhibiting prostaglandin (PG)E2, 8-iso-PGF2α and serotonin (5-HT) levels, as well as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and nitric oxide synthase gene expression in colon specimens of DSS-induced colitis. Furthermore, MIA-690 decreased serum insulin-like growth factor (IGF)-1 levels in mice DSS-treated, respect to MR-409. Thus, our findings highlight the protective effects of MIA-690 and MR-409 on inflammation stimuli. The higher antinflammatory and antioxidant activities observed with MIA-690 could be related to decreased serum IGF-1 levels. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a450469d8d65d1db85e9632944b7d05Test https://doi.org/10.1038/s41598-021-81778-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1a450469d8d65d1db85e9632944b7d05 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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