A syntaxin 10–SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells
| العنوان: | A syntaxin 10–SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells |
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| المؤلفون: | Eric J. Espinosa, Ian G. Ganley, Suzanne R. Pfeffer |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | The Rockefeller University Press, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Cholera Toxin, Endosome, Endocytic cycle, Endosomes, Syntaxin 16, Biology, Binding, Competitive, Article, Receptor, IGF Type 2, Cell Line, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Syntaxin, Humans, Research Articles, 030304 developmental biology, 0303 health sciences, Membrane Glycoproteins, Qa-SNARE Proteins, Golgi Matrix Proteins, Membrane Proteins, Cell Biology, Golgi apparatus, Transport protein, Cell biology, Protein Transport, rab GTP-Binding Proteins, symbols, Rab, Soluble NSF attachment protein, SNARE complex, SNARE Proteins, 030217 neurology & neurosurgery, trans-Golgi Network |
| الوصف: | Mannose 6-phosphate receptors (MPRs) are transported from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway. This process requires Rab9 guanosine triphosphatase (GTPase) and the putative tether GCC185. We show in human cells that a soluble NSF attachment protein receptor (SNARE) complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport but not for the STX6-dependent transport of TGN46 or cholera toxin from early endosomes to the Golgi. Depletion of STX10 leads to MPR missorting and hypersecretion of hexosaminidase. Mouse and rat cells lack STX10 and, thus, must use a different target membrane SNARE for this process. GCC185 binds directly to STX16 and is competed by Rab6. These data support a model in which the GCC185 tether helps Rab9-bearing transport vesicles deliver their cargo to the trans-Golgi and suggest that Rab GTPases can regulate SNARE–tether interactions. Importantly, our data provide a clear molecular distinction between the transport of MPRs and TGN46 to the trans-Golgi. |
| اللغة: | English |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18c638876d6b4fa0e776f7db3ee076d6Test http://europepmc.org/articles/PMC2213607Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....18c638876d6b4fa0e776f7db3ee076d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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