Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1
| العنوان: | Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1 |
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| المؤلفون: | Liang Yuan, Dajie Wang, Chunyang Wu |
| المصدر: | Bioengineered, Vol 13, Iss 2, Pp 2840-2850 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell Survival, sirt1, proliferation, Myocytes, Smooth Muscle, Bioengineering, Coronary Disease, Protective Agents, Applied Microbiology and Biotechnology, Muscle, Smooth, Vascular, Glucosides, Sirtuin 1, Humans, coronary heart disease, Cells, Cultured, Cell Proliferation, hvsmcs, liquiritin, lactate dehydrogenase, General Medicine, Atherosclerosis, Up-Regulation, Lipoproteins, LDL, Gene Expression Regulation, Cytoprotection, Flavanones, TP248.13-248.65, Biotechnology |
| الوصف: | This study aimed to explore whether liquiritin affects the development of coronary heart disease by regulating the proliferation and migration of human vascular smooth muscle cells (hVSMCs). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release detection were performed to measure the toxic effects of liquiritin on hVSMCs. An in vitro atherosclerosis model in hVSMCs was established using oxidized low-density lipoprotein (ox-LDL), and cell proliferation and apoptosis were detected using an MTT assay and flow cytometry analysis. Western blotting and reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) were used to detect protein and mRNA expressions, respectively. Caspase3 activity and cell migration were measured using an activity detection kit and Transwell assay, respectively. The results indicated that liquiritin at doses |
| اللغة: | English |
| تدمد: | 2165-5987 2165-5979 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17d80677f95a03a4962386a601418a0fTest https://doaj.org/article/05762d0021dc438aa0d8b22d72b30a29Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....17d80677f95a03a4962386a601418a0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21655987 21655979 |
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