The Bacterial Product Violacein Exerts an Immunostimulatory Effect Via TLR8
| العنوان: | The Bacterial Product Violacein Exerts an Immunostimulatory Effect Via TLR8 |
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| المؤلفون: | Andreas Kaufmann, Gabriele Köllisch, Alfonso J. García-Piñeres, Wibke E. Diederich, Stefan Bauer, Juan J. Araya, Kerstin Mark, Francisco A. Venegas, Max Chavarría |
| المصدر: | Scientific Reports, vol.9(13661), pp.1-17 Kérwá Universidad de Costa Rica instacron:UCR Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Models, Molecular, 0301 basic medicine, Indoles, Protein Conformation, THP-1 Cells, Interleukin-1beta, lcsh:Medicine, Peripheral blood mononuclear cell, Article, Immunostimulatory, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Target identification, Animals, Humans, Secretion, lcsh:Science, Transcriptomics, Monocytes and macrophages, Multidisciplinary, biology, Assay systems, Interleukin-6, Chemistry, Chromobacterium, lcsh:R, Bacterial, TLR7, TLR8, biology.organism_classification, Cell biology, Imidazoquinoline, HEK293 Cells, RAW 264.7 Cells, 030104 developmental biology, Gene Expression Regulation, Toll-Like Receptor 8, Cell culture, 030220 oncology & carcinogenesis, Leukocytes, Mononuclear, lcsh:Q, Chromobacterium violaceum, Signal Transduction, Violacein |
| الوصف: | Violacein, an indole-derived, purple-colored natural pigment isolated from Chromobacterium violaceum has shown multiple biological activities. In this work, we studied the effect of violacein in different immune cell lines, namely THP-1, MonoMac 6, ANA-1, Raw 264.7 cells, as well as in human peripheral blood mononuclear cells (PBMCs). A stimulation of TNF-α production was observed in murine macrophages (ANA-1 and Raw 264.7), and in PBMCs, IL-6 and IL-1β secretion was detected. We obtained evidence of the molecular mechanism of activation by determining the mRNA expression pattern upon treatment with violacein in Raw 264.7 cells. Incubation with violacein caused activation of pathways related with an immune and inflammatory response. Our data utilizing TLR-transfected HEK-293 cells indicate that violacein activates the human TLR8 (hTLR8) receptor signaling pathway and not human TLR7 (hTLR7). Furthermore, we found that the immunostimulatory effect of violacein in PBMCs could be suppressed by the specific hTLR8 antagonist, CU-CPT9a. Finally, we studied the interaction of hTLR8 with violacein in silico and obtained evidence that violacein could bind to hTLR8 in a similar fashion to imidazoquinoline compounds. Therefore, our results indicate that violacein may have some potential in contributing to future immune therapy strategies. Universidad de Costa Rica/[801-B2-519]/UCR/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[FI-497-11]/MICITT/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[DFG-TR84]/MICITT/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[DFG-KFO325]/MICITT/Costa Rica UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Química UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA) UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM) UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones Farmacéuticas (INIFAR) |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c8b8424799dcfa1237231a5b8bb2a2Test https://doi.org/10.1038/s41598-019-50038-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....17c8b8424799dcfa1237231a5b8bb2a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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