Next-Generation Autoantibody Testing by Combination of Screening and Confirmation—the CytoBead® Technology
| العنوان: | Next-Generation Autoantibody Testing by Combination of Screening and Confirmation—the CytoBead® Technology |
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| المؤلفون: | Dirk Reinhold, Dirk Roggenbuck, Mandy Sowa, Karsten Conrad, Rico Hiemann, Peter Schierack |
| المصدر: | Clinical Reviews in Allergy & Immunology |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Anti-nuclear antibody, Second-generation autoantibody testing, Autoimmunity, Context (language use), Multiplex diagnostics, Article, Autoimmune Diseases, 03 medical and health sciences, 0302 clinical medicine, Autoimmune disease, Humans, Immunology and Allergy, Medicine, Fluorescent Antibody Technique, Indirect, Digital fluorescence, Autoantibodies, 030203 arthritis & rheumatology, Indirect immunofluorescence, business.industry, Autoantibody, IIf, General Medicine, Clinical routine, 030104 developmental biology, Antibodies, Antinuclear, Immunology, business, Biomarkers |
| الوصف: | Occurrence of autoantibodies (autoAbs) is a hallmark of autoimmune diseases, and the analysis thereof is an essential part in the diagnosis of organ-specific autoimmune and systemic autoimmune rheumatic diseases (SARD), especially connective tissue diseases (CTDs). Due to the appearance of autoAb profiles in SARD patients and the complexity of the corresponding serological diagnosis, different diagnostic strategies have been suggested for appropriate autoAb testing. Thus, evolving assay techniques and the continuous discovery of novel autoantigens have greatly influenced the development of these strategies. Antinuclear antibody (ANA) analysis by indirect immunofluorescence (IIF) on tissue and later cellular substrates was one of the first tests introduced into clinical routine and is still an indispensable tool for CTD serology. Thus, screening for ANA by IIF is recommended to be followed by confirmatory testing of positive findings employing different assay techniques. Given the continuous growth in the demand for autoAb testing, IIF has been challenged as the standard method for ANA and other autoAb analyses due to lacking automation, standardization, modern data management, and human bias in IIF pattern interpretation. To address these limitations of autoAb testing, the CytoBead® technique has been introduced recently which enables automated interpretation of cell-based IIF and quantitative autoAb multiplexing by addressable microbead immunoassays in one reaction environment. Thus, autoAb screening and confirmatory testing can be combined for the first time. The present review discusses the history of autoAb assay techniques in this context and gives an overview and outlook of the recent progress in emerging technologies. |
| تدمد: | 1559-0267 1080-0549 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16cb407522944a38ad71d7c58adf3d58Test https://doi.org/10.1007/s12016-016-8574-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....16cb407522944a38ad71d7c58adf3d58 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15590267 10800549 |
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