Functional characterization of GPC-1 genes in hexaploid wheat
| العنوان: | Functional characterization of GPC-1 genes in hexaploid wheat |
|---|---|
| المؤلفون: | Raz Avni, Facundo Tabbita, Stephen Pearce, Assaf Distelfeld, Rongrong Zhao, Jorge Dubcovsky, Tzion Fahima, Cristobal Uauy, Yan Jun, Ann J. Slade |
| المصدر: | Avni, R; Zhao, R; Pearce, S; Jun, Y; Uauy, C; Tabbita, F; et al.(2014). Functional characterization of GPC-1 genes in hexaploid wheat.. Planta, 239(2), 313-324. doi: 10.1007/s00425-013-1977-y. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/1sd065mzTest Planta, vol 239, iss 2 |
| بيانات النشر: | Springer, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Chlorophyll, GRAIN PROTEIN CONTENT, Time Factors, Mutant, Plant Biology, Plant Science, Remobilization, chemistry.chemical_compound, Gene Knockout Techniques, Nutrient, Gene Expression Regulation, Plant, Biomass, Micronutrients, Photosynthesis, Triticum, Plant Proteins, education.field_of_study, food and beverages, Plants, Plants, Genetically Modified, Horticulture, Zinc, Phenotype, Ethyl Methanesulfonate, Biotecnología Agrícola y Biotecnología Alimentaria, Senescence, Crop and Pasture Production, Ethyl methanesulfonate, Nitrogen, NAM transcription factor, Iron, Population, Plant Biology & Botany, Biotecnología Agropecuaria, WHEAT, Genetically Modified, Biology, Article, Anthesis, Botany, Genetics, education, Gene, Cloning, Biological Transport, Plant, Plant Leaves, chemistry, Gene Expression Regulation, MUTANTS, SENESCENCE, CIENCIAS AGRÍCOLAS, Mutation, Edible Grain, Transcription Factors |
| الوصف: | In wheat, monocarpic senescence is a tightly regulated process during which nitrogen (N) and micronutrients stored pre-anthesis are remobilized from vegetative tissues to the developing grains. Recently, a close connection between senescence and remobilization was shown through the map-based cloning of the GPC (grain protein content) gene in wheat. GPC-B1 encodes a NAC transcription factor associated with earlier senescence and increased grain protein, iron and zinc content, and is deleted or non-functional in most commercial wheat varieties. In the current research, we identified 'loss of function' ethyl methanesulfonate mutants for the two GPC-B1 homoeologous genes; GPC-A1 and GPC-D1, in a hexaploid wheat mutant population. The single gpc-a1 and gpc-d1 mutants, the double gpc-1 mutant and control lines were grown under field conditions at four locations and were characterized for senescence, GPC, micronutrients and yield parameters. Our results show a significant delay in senescence in both the gpc-a1 and gpc-d1 single mutants and an even stronger effect in the gpc-1 double mutant in all the environments tested in this study. The accumulation of total N in the developing grains showed a similar increase in the control and gpc-1 plants until 25 days after anthesis (DAA) but at 41 and 60 DAA the control plants had higher grain N content than the gpc-1 mutants. At maturity, GPC in all mutants was significantly lower than in control plants while grain weight was unaffected. These results demonstrate that the GPC-A1 and GPC-D1 genes have a redundant function and play a major role in the regulation of monocarpic senescence and nutrient remobilization in wheat. Fil: Avni, Raz. Tel Aviv University; Israel Fil: Zhao, Rongrong. China Agricultural University; República de China. University of California at Davis; Estados Unidos Fil: Pearce, Stephen. University of California at Davis; Estados Unidos Fil: Jun, Yan. University of Haifa; Israel Fil: Uauy, Cristobal. University of California at Davis; Estados Unidos Fil: Tabbita, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Davis; Estados Unidos Fil: Fahima, Tzion. University of Haifa; Israel Fil: Slade, Ann. Arcadia Biosciences Inc.; Estados Unidos Fil: Dubcovsky, Jorge. University of California at Davis; Estados Unidos Fil: Distelfeld, Assaf. Tel Aviv University; Israel |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::161e639fca20a7cdd370bed653fdbcdeTest https://link.springer.com/article/10.1007/s00425-013-1977-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....161e639fca20a7cdd370bed653fdbcde |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |