Valsartan impedes epinephrine-induced ICAM-4 activation on normal, sickle cell trait and sickle cell disease red blood cells
| العنوان: | Valsartan impedes epinephrine-induced ICAM-4 activation on normal, sickle cell trait and sickle cell disease red blood cells |
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| المؤلفون: | Sasia-Marie Jones, Biree Andemariam, Jing Zhang, George Lykotrafitis |
| المصدر: | PLoS ONE PLoS ONE, Vol 14, Iss 5, p e0216467 (2019) |
| بيانات النشر: | Public Library of Science, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cell, Cell Membranes, 030204 cardiovascular system & hematology, Microscopy, Atomic Force, Biochemistry, Intracellular Receptors, Mice, 0302 clinical medicine, Catecholamines, Cell Signaling, Chlorocebus aethiops, Medicine and Health Sciences, Membrane Receptor Signaling, Amines, Receptor, Multidisciplinary, Chemistry, Organic Compounds, Neurotransmitter Receptor Signaling, Neurochemistry, Neurotransmitters, Hematology, 3. Good health, Spring, medicine.anatomical_structure, Epinephrine, Valsartan, Genetic Diseases, Physical Sciences, COS Cells, Medicine, Female, Seasons, Cellular Structures and Organelles, Intracellular, medicine.drug, Research Article, Signal Transduction, Adult, medicine.medical_specialty, Biogenic Amines, Transmembrane Receptors, Adolescent, Science, Erythrocytes, Abnormal, Receptor, Angiotensin, Type 1, Sickle Cell Trait, 03 medical and health sciences, Autosomal Recessive Diseases, Internal medicine, medicine, Animals, Humans, Clinical Genetics, Sickle cell trait, Sickle Cell Disease, Organic Chemistry, Erythrocyte Membrane, Chemical Compounds, Biology and Life Sciences, Proteins, Cell Biology, medicine.disease, Angiotensin II, Hormones, Hemoglobinopathies, Red blood cell, 030104 developmental biology, Endocrinology, HEK293 Cells, Earth Sciences, G Protein Coupled Receptors, Cell Adhesion Molecules, Neuroscience |
| الوصف: | Abnormal red blood cell (RBC) adhesion to endothelial αvβ3 plays a crucial role in triggering vaso-occlusive episodes in sickle cell disease (SCD). It is known that epinephrine, a β-adrenergic receptor (β-AR) stimulator, increases the RBC surface density of active intercellular adhesion molecule-4 (ICAM-4) which binds to the endothelial αvβ3. It has also been demonstrated that in human embryonic kidney 293 cells, mouse cardiomyocytes, and COS-7 cell lines, the β-adrenergic and renin-angiotensin systems are interrelated and that there is a direct interaction and cross-regulation between β-AR and angiotensin II type 1 receptor (AT1R). Selective blockade of AT1R reciprocally inhibits the downstream signaling of β-ARs, similar to the inhibition observed in the presence of a β-AR-blocker. However, it is not known if this mechanism is active in human RBCs. Here, we studied the effect of valsartan, an AT1R blocker, on the surface density of active ICAM-4 receptors in normal, sickle cell trait, and homozygous sickle RBCs. We applied single molecule force spectroscopy to detect active ICAM-4 receptors on the RBC plasma membrane with and without the presence of valsartan and epinephrine. We found that epinephrine significantly increased whereas valsartan decreased their surface density. Importantly, we found that pretreatment of RBCs with valsartan significantly impeded the activation of ICAM-4 receptors induced by epinephrine. The observed reduced expression of active ICAM-4 receptors on the RBC plasma membrane leads us to conjecture that valsartan may be used as a supporting remedy for the prevention and treatment of vaso-occlusive crisis in SCD. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15c6a15af8ea5de99084afa21a8f2c1fTest http://europepmc.org/articles/PMC6513067Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....15c6a15af8ea5de99084afa21a8f2c1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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