Multiple calmodulin genes of the Pacific abalone, Haliotis discus hannai (Mollusca: Vetigastropoda: Haliotidae)
| العنوان: | Multiple calmodulin genes of the Pacific abalone, Haliotis discus hannai (Mollusca: Vetigastropoda: Haliotidae) |
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| المؤلفون: | Tae Hyug Jeong, Jong Kyu Lee, Han Kyu Lim, Gun-Do Kim |
| المصدر: | Animal Cells and Systems, Vol 22, Iss 5, Pp 341-351 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Untranslated region, Calmodulin, Abalone, invertebrate, calcium signaling, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Haliotis discus, Gene, Mollusca, lcsh:QH301-705.5, Genetics, lcsh:R5-920, Vetigastropoda, biology, gene structure, biology.organism_classification, Gene expression profiling, 030104 developmental biology, expression profiling, lcsh:Biology (General), biology.protein, Animal Science and Zoology, lcsh:Medicine (General) |
| الوصف: | In this study, we identified four canonical calmodulin genes in the Pacific abalone, Haliotis discus hannai. Their full-length cDNAs were variable in the 5′ and 3′ untranslated regions, but highly similar (91–97%) in the coding region. Each of the genes encoded 149 amino acids, with 93–97% similarity among themselves and 94–98% similarity with human CAM I. There were 54 substitutions distributed unevenly throughout the coding regions, found mostly in the third codon position. Gene structure analysis revealed that each of the calmodulin genes comprised five exons and four introns. The intron positions and phases were identical and there were no introns in the fourth exon. The corresponding introns differed in their sequences and sizes. Expression profiles of nine tissues from abalone revealed that the calmodulin genes were transcribed in common in gill and mantle tissue, but differentially in the other tissues. A phylogenetic analysis based on the amino acid sequences revealed that calmodulin C was the most common isoform in Gastropoda and calmodulin was the most diverged isoform. An in silico analysis of the calmodulin genes identified paralogous genes in other Haliotis species, indicating that gene duplication might have occurred in the last common ancestor of Haliotis. Abbreviations: ORF: open reading frame; RACE: random amplification of cDNA end; TSA: transcriptome shotgun assembly; UTR: untranslated region |
| اللغة: | English |
| تدمد: | 2151-2485 1976-8354 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15292e8baa8fa6b2fbd0d5b3ebad4180Test https://doaj.org/article/381ee113dcc74cc998b582857c9ec4aeTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....15292e8baa8fa6b2fbd0d5b3ebad4180 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21512485 19768354 |
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