Retracted : Cav1.3 is upregulated in osteoporosis rat model and promotes osteoclast differentiation from preosteoclast cell line RAW264.7
| العنوان: | Retracted : Cav1.3 is upregulated in osteoporosis rat model and promotes osteoclast differentiation from preosteoclast cell line RAW264.7 |
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| المؤلفون: | Yining Sun, Wujun Xue, Nan Hu, Xiaohong Lv, Dan Pu, Lan He, Ping Fan, Zhiming Hao, Yang Li, Xiuyuan Feng |
| المصدر: | Journal of Cellular Physiology. 234:12821-12827 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | musculoskeletal diseases, 0301 basic medicine, Calcium Channels, L-Type, Physiology, Cellular differentiation, Clinical Biochemistry, Osteoporosis, Osteoclasts, Bone resorption, Mice, 03 medical and health sciences, 0302 clinical medicine, Western blot, Downregulation and upregulation, Osteoclast, medicine, Animals, medicine.diagnostic_test, biology, Chemistry, Acid phosphatase, Cell Differentiation, Cell Biology, medicine.disease, Rats, Up-Regulation, Cell biology, RAW 264.7 Cells, 030104 developmental biology, medicine.anatomical_structure, Gene Expression Regulation, RANKL, 030220 oncology & carcinogenesis, biology.protein, Female, RNA Interference, Calcium Channels |
| الوصف: | Background Osteoporosis (OP) is a systemic osteopathy with increased bone fragility and increased risk of fracture. Osteoclasts (OC) are the key target cells in the treatment of osteoporosis. We aimed to research the role of L-type calcium channel protein Cav1.3 in OC differentiation in this study. Methods OP rat model was established to detect the expression level of Cav1.3. Tartrate-resistant acid phosphatase assay was used to measure the differentiation of osteoclast during receptor activator of nuclear factor κ-Β ligand (RANKL)-induced osteoclasts formation. The expression of bone differentiation-related proteins were detected by western blot analysis. Results Cav1.3 is upregulated in OP rats. Knockdown of Cav1.3 inhibits the differentiation of RAW264.7. Cav1.3 regulates the cell differentiation and bone resorption of RAW264.7 during RANKL-induced osteoclasts formation, which is accompanied by upregulation of CaMK II, p-CERB, AP-1, NFATC1, and NF-κB. Conclusion Cav1.3 plays an important role in osteoporosis and the differentiation of osteoclast, which might be involved with the bone differentiation-related proteins. |
| تدمد: | 1097-4652 0021-9541 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1524e27758f44c73bddb6fdc394fe975Test https://doi.org/10.1002/jcp.27937Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1524e27758f44c73bddb6fdc394fe975 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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