Discovery of Dehydroamino Acid Residues in the Capsid and Matrix Structural Proteins of HIV-1
| العنوان: | Discovery of Dehydroamino Acid Residues in the Capsid and Matrix Structural Proteins of HIV-1 |
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| المؤلفون: | Rachel M. Miller, Rachel A. Knoener, Bayleigh E. Benner, Brian L. Frey, Mark Scalf, Michael R. Shortreed, Nathan M. Sherer, Lloyd M. Smith |
| المصدر: | J Proteome Res |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Proteomics, Capsid, HIV-1, Virion, Humans, Capsid Proteins, General Chemistry, Biochemistry, Article |
| الوصف: | Human immunodeficiency virus type 1 (HIV-1) remains a deadly infectious disease despite existing antiretroviral therapies. A comprehensive understanding of the specific mechanisms of viral infectivity remains elusive and currently limits the development of new and effective therapies. Through in-depth proteomic analysis of HIV-1 virions, we discovered the novel post-translational modification of highly conserved residues within the viral matrix and capsid proteins to the dehydroamino acids, dehydroalanine and dehydrobutyrine. We further confirmed their presence by labeling the reactive alkene, characteristic of dehydroamino acids, with glutathione via Michael addition. Dehydroamino acids are rare, understudied, and have been observed mainly in select bacterial and fungal species. Until now, they have not been observed in HIV proteins. We hypothesize that these residues are important in viral particle maturation and could provide valuable insight into HIV infectivity mechanisms. |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1455a18ed0c3f83ed19bcae11ee6cb1dTest https://doi.org/10.1021/acs.jproteome.1c00867Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1455a18ed0c3f83ed19bcae11ee6cb1d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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