Signature of Gut Microbiome by Conventional and Advanced Analysis Techniques: Advantages and Disadvantages
| العنوان: | Signature of Gut Microbiome by Conventional and Advanced Analysis Techniques: Advantages and Disadvantages |
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| المؤلفون: | Asadzadeh Aghdaei H, Ahmadi Bashirzadeh D, Nazemalhosseini Mojarad E, Rezasoltani S, Shabnam Shahrokh, Mohsen Norouzinia |
| المصدر: | Middle East Journal of Digestive Diseases |
| بيانات النشر: | Iranian Association of Gastroerterology and Hepatology, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbial diversity, Computational biology, Review Article, Gut microbiota, Gut flora, digestive system, 03 medical and health sciences, Taxonomic composition, Human health, 0302 clinical medicine, Microbiome analysis techniques, Medicine, Microbiome, Hepatology, biology, business.industry, digestive, oral, and skin physiology, Gastroenterology, biology.organism_classification, Disadvantages, Gut microbiome, 030104 developmental biology, Metagenomics, 030220 oncology & carcinogenesis, Amplicon sequencing, Advantages, business |
| الوصف: | Gut microbiota is considered as a human organ with its own specific functions and complexity. Development of novel techniques such as shut gun sequencing, metagenomics, and next-generation sequencing (NGS) has enabled bypassing the traditional culturedependent bias and has significantly expanded our understanding of the composition, diversity, and roles of the gut microbiota in human health and diseases. Although amplicon sequencing characterizes the taxonomic composition of the gut microbiome, it is impossible to cover the direct evidence of the microbial biological functions related to the gut microbial community. Hence, the critical next step for gut microbiome studies is shifting from gene/ genome-centric analysis to mechanism-centric techniques by integrating omics data with experimental results. Realizing gut microbial diversity and their bioactive metabolites function will provide insight into the clinical application of gut microbiota in diagnoses and treatments of several diseases. In this review, we focused on explaining the conventional and advanced microbiome analysis techniques regarding gut microbiota investigation with considering the advantages and disadvantages of the platforms. |
| اللغة: | English |
| تدمد: | 2008-5249 2008-5230 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13757ae971fcabf3a2878a2e2df60841Test http://europepmc.org/articles/PMC7023644Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....13757ae971fcabf3a2878a2e2df60841 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20085249 20085230 |
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