The utility of high‐risk human papillomavirus in situ hybridization in cytology cell block material from cystic head and neck lesions
| العنوان: | The utility of high‐risk human papillomavirus in situ hybridization in cytology cell block material from cystic head and neck lesions |
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| المؤلفون: | Sarah M. Calkins, Tara A. Saunders, Lucy M. Han |
| المصدر: | Cancer Cytopathology. 130:154-160 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, In situ hybridization, Alphapapillomavirus, Metastasis, hemic and lymphatic diseases, Cytology, Biomarkers, Tumor, medicine, False positive paradox, Humans, Papillomaviridae, neoplasms, Lymph node, Cyclin-Dependent Kinase Inhibitor p16, In Situ Hybridization, Cell block, P16 immunohistochemistry, business.industry, Papillomavirus Infections, virus diseases, medicine.disease, medicine.anatomical_structure, Oncology, Head and Neck Neoplasms, DNA, Viral, Carcinoma, Squamous Cell, Immunohistochemistry, business |
| الوصف: | BACKGROUND Human papillomavirus-related oropharyngeal squamous cell carcinoma (HPV-OPSCC) presents frequently as metastasis in a neck lymph node that may be cystic or necrotic. Fine-needle aspiration (FNA) biopsies are often first-line diagnostic procedures. p16 immunohistochemistry (IHC) is a surrogate marker for high-risk HPV (hrHPV) infection but can be challenging to interpret. This study evaluated the use of hrHPV in situ hybridization (ISH) in cytology cell blocks of cystic neck lesions. METHODS Twenty-four FNA cases with cell blocks and surgical correlates were evaluated. p16 IHC and hrHPV ISH were assessed on cell blocks (C-p16 and C-hrHPV ISH), and hrHPV ISH on surgical samples (S-hrHPV ISH). All results were classified as negative, positive, or equivocal. RESULTS Two cases were excluded because of insufficient tissue on recut. On the basis of C-hrHPV ISH cases, 12 were positive, 5 were negative, and 5 were equivocal. All 12 positive C-hrHPV ISH cases had concordant S-hrHPV ISH with no false positives. Of the 5 negative C-hrHPV ISH cases, 4 had concordant S-hrHPV ISH, and 1 had a discordant S-hrHPV ISH. Of the 5 equivocal C-hrHPV ISH cases, S-hrHPV ISH were both positive and negative. Fourteen cases were equivocal by C-p16; 9 cases were reliably classified by C-hrHPV ISH (5 positive, 4 negative; 64%). CONCLUSIONS C-hrHPV ISH can be reliably used, especially when positive. A negative or equivocal interpretation of C-hrHPV ISH may warrant repeat testing. Compared to C-p16, C-hrHPV ISH is more frequently diagnostic and could be helpful for HPV-OSCC diagnosis and management. |
| تدمد: | 1934-6638 1934-662X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::125bfedaedba15dfb1b59bc67945fb96Test https://doi.org/10.1002/cncy.22529Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....125bfedaedba15dfb1b59bc67945fb96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19346638 1934662X |
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