Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models
| العنوان: | Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models |
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| المؤلفون: | Eun-Ji Song, Na Rae Shin, Young-Do Nam, Han Seok Choi, Hee-Bok Kim, Dong-Woo Lim, Yun Sung Lim, Jing-Hua Wang, Hojun Kim, Shambhunath Bose |
| المصدر: | The FASEB Journal. 34:8686-8701 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, endocrine system, medicine.medical_specialty, endocrine system diseases, medicine.medical_treatment, Population, Thyroid Gland, Levothyroxine, Physiology, Biology, Prevotellaceae, Gut flora, Biochemistry, Rats, Sprague-Dawley, Feces, 03 medical and health sciences, 0302 clinical medicine, Hypothyroidism, RNA, Ribosomal, 16S, Internal medicine, Genetics, medicine, Animals, education, Molecular Biology, education.field_of_study, Bacteria, Bacteroidetes, business.industry, Microbiota, Thyroid, Thyroidectomy, biology.organism_classification, Gastrointestinal Microbiome, Rats, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Metagenomics, Propylthiouracil, Thyroid function, business, 030217 neurology & neurosurgery, Biotechnology, medicine.drug |
| الوصف: | Background: The role of gut microbiota in metabolic diseases has recently been widely verified. Thyroid hormones are essential for the regulation of energy homeostasis and metabolic processes. However, the relationship between thyroid function and host gut microbial communities is not properly understood. Method: To determine whether and how gut microbiota is associated with thyroid function, metagenomics analysis of the bacterial population in fecal samples of rat models of hyperthyroidism (induced by levothyroxine) and hypothyroidism (induced by propylthiouracil or thyroidectomy) was conducted through 16SrRNA gene sequencing. Finding: Our results revealed that all thyroid dysfunction models were definitely established and gut microbial composition varied according to different thyroid functional status. The relative abundance of Ruminococcus was significantly higher in the hyperthyroidism group (HE) vs both the normal and hypothyroidism (HO) groups while S24-7 was significantly higher in the HO group compared to both the normal and HE groups. The relative abundance of Prevotellaceae and Prevotella were significantly lower in the HO group vs the normal. Firmicutes and Oscillospira was significantly higher in the SHO (surgery-induced hypothyroidism) group than the SHAM group, while Prevotellaceae andPrevotella showed lower abundance in the SHO group compared to the SHAM group. Interpretation: This is the first perspective study of the relationship between thyroid gland and gut microbiota using Next Generation Sequencing-based microbial identification technology. We demonstrated that thyroid status and function influenced the gut microbial composition, and the results presented here can be used as reference in future studies to identify potential mechanism(s) by which thyroid modulates gut microbial population. Funding Statement: This work was supported by a National Research Foundation (NRF) of Korea Grant funded by the Korean Government (NRF- 2019R1A2B5B01070365). This work was also supported by the Main Research Program (E0170602-02) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science and ICT. Declaration of Interests: The authors declare no conflict of interest. Ethics Approval Statement: The animal experimental protocol was approved by the Institutional Animal Care and Use Committee of Dongguk University (approval no. 2015-08128) and all experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals |
| تدمد: | 1530-6860 0892-6638 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::113e6a1904506b52f19bc83ba749b8daTest https://doi.org/10.1096/fj.201903091rrTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....113e6a1904506b52f19bc83ba749b8da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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