Routine blood tests as a potential diagnostic tool for COVID-19
| العنوان: | Routine blood tests as a potential diagnostic tool for COVID-19 |
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| المؤلفون: | Davide Ferrari, Marta Strollo, Giuseppe Banfi, Andrea Motta, Massimo Locatelli |
| المساهمون: | Ferrari, D., Motta, A., Strollo, M., Banfi, G., Locatelli, M. |
| المصدر: | Clinical Chemistry and Laboratory Medicine (CCLM). 58:1095-1099 |
| بيانات النشر: | Walter de Gruyter GmbH, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Clinical Biochemistry, blood test, 030218 nuclear medicine & medical imaging, chemistry.chemical_compound, 0302 clinical medicine, aspartate aminotransferase, Leukocytes, Medicine, Gamma-glutamyltransferase, Aged, 80 and over, 0303 health sciences, Hematologic Tests, biology, medicine.diagnostic_test, Alanine Transaminase, gamma-Glutamyltransferase, General Medicine, Middle Aged, C-Reactive Protein, Italy, Absolute neutrophil count, RNA, Viral, Female, Coronavirus Infections, Adult, Blood Platelets, medicine.medical_specialty, Pneumonia, Viral, RT-PCR, WBC, Real-Time Polymerase Chain Reaction, Betacoronavirus, 03 medical and health sciences, Lactate dehydrogenase, Internal medicine, Humans, Blood test, Aspartate Aminotransferases, Pandemics, Aged, Retrospective Studies, 030304 developmental biology, L-Lactate Dehydrogenase, SARS-CoV-2, business.industry, Biochemistry (medical), C-reactive protein, COVID-19, lactate dehydrogenase, Gold standard (test), Alkaline Phosphatase, Alanine transaminase, chemistry, biology.protein, Laboratories, business, Biomarkers, Blood sampling |
| الوصف: | Objectives The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to date, the epidemic has gradually spread to 209 countries worldwide with more than 1.5 million infected people and 100,000 deaths. Amplification of viral RNA by rRT-PCR serves as the gold standard for confirmation of infection, yet it needs a long turnaround time (3–4 h to generate results) and shows false-negative rates as large as 15%–20%. In addition, the need of certified laboratories, expensive equipment and trained personnel led many countries to limit the rRT-PCR tests only to individuals with pronounced respiratory syndrome symptoms. Thus, there is a need for alternative, less expensive and more accessible tests. Methods We analyzed the plasma levels of white blood cells (WBCs), platelets, C-reactive protein (CRP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transpeptidase (GGT), alkaline phosphatase and lactate dehydrogenase (LDH) of 207 patients who, after being admitted to the emergency room of the San Raffaele Hospital (Milan, Italy) with COVID-19 symptoms, were rRT-PCR tested. Of them, 105 tested positive, whereas 102 tested negative. Results Statistically significant differences were observed for WBC, CRP, AST, ALT and LDH. Empirical thresholds for AST and LDH allowed the identification of 70% of either COVID-19-positive or -negative patients on the basis of routine blood test results. Conclusions Combining appropriate cutoffs for certain hematological parameters could help in identifying false-positive/negative rRT-PCR tests. Blood test analysis might be used as an alternative to rRT-PCR for identifying COVID-19-positive patients in those countries which suffer from a large shortage of rRT-PCR reagents and/or specialized laboratory. |
| تدمد: | 1437-4331 1434-6621 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::109ff33594938b457669297d4f81a00dTest https://doi.org/10.1515/cclm-2020-0398Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....109ff33594938b457669297d4f81a00d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14374331 14346621 |
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