Neurotrophin regulation of sodium and calcium channels in human neuroblastoma cells
| العنوان: | Neurotrophin regulation of sodium and calcium channels in human neuroblastoma cells |
|---|---|
| المؤلفون: | F. J. Urbano, Washinton Buño |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Elsevier, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | medicine.medical_specialty, Time Factors, Sodium Channels, Neuroblastoma, Neurotrophic factors, Internal medicine, Nerve Growth Factor, Tumor Cells, Cultured, medicine, Humans, Brain-derived neurotrophic factor, biology, Voltage-dependent calcium channel, Brain Neoplasms, Chemistry, Brain-Derived Neurotrophic Factor, General Neuroscience, medicine.disease, Cell biology, Nerve growth factor Brain-derived neurotrophic factor Tetrodotoxin sensitivity Neuronal differentiation Ionic channel expression Cell culture, Endocrinology, Nerve growth factor, biology.protein, Tumor necrosis factor alpha, Calcium Channels, Tyrosine kinase, Neurotrophin |
| الوصف: | Neurotrophins, acting through tyrosine kinase family genes, are essential for neuronal differentiation. The expression of tyrosine kinase family genes is prognostic in neuroblastoma, and neurotrophins reduce proliferation and induce differentiation, indicating that neuroblastomas are regulated by neurotrophins. We tested the effects of nerve growth factor and brain-derived neurotrophic factor on Na+ and Ca2+ currents, using the whole-cell patch-clamp technique, in human neuroblastoma NB69 cells. Control cells exhibited a slow tetrodotoxin-resistant (IC50=98 nM) Na+ current and a high-voltage-activated Ca2+ current. Exposure to nerve growth factor (50 ng/ml) and/or brain-derived neurotrophic factor (5 ng/ml) produced the expression of a fast tetrodotoxin-sensitive (IC50=10 nM) Na+ current after day 3, and suppressed the slow tetrodotoxin-resistant variety. The same type of high-voltage-activated Ca2+ current was expressed in control and treated cells. The treatment increased the surface density of both Na+ and Ca2+ currents with time after plating, from 17 pA/pF at days 3-5 and 1-5 to 34 and 30 pA/pF after days 6-10, respectively. Therefore, both nerve growth factor and brain-derived neurotrophic factor, acting through different receptors of the tyrosine kinase family and also possibly the tumor necrosis factor receptor-II, were able to regulate differentiation and the expression of Na+ and Ca2+ channels, partially reproducing the modifications induced by diffusible astroglial factors. We show that neurotrophins induced differentiation to a neuronal phenotype and modified the expression of Na+ and Ca2+ currents, partially reproducing the effects of diffusible astroglial factors. (C) 2000 IBRO. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fced492a32708b11bda5a7ed7a728c8Test http://hdl.handle.net/10261/152387Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0fced492a32708b11bda5a7ed7a728c8 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |