Genome-wide gene expression array identifies novel genes related to disease severity and excessive daytime sleepiness in patients with obstructive sleep apnea
| العنوان: | Genome-wide gene expression array identifies novel genes related to disease severity and excessive daytime sleepiness in patients with obstructive sleep apnea |
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| المؤلفون: | Chung-Jen Chen, Yi Xin Zheng, Chang-Chun Hsiao, Mao Chang Su, Jen Chieh Chang, Kuo Tung Huang, Chien Hung Chin, Meng-Chih Lin, Kuang Den Chen, Ting Ya Wang, Ting-Wen Chen, Yung-Che Chen, Chia Wei Liou, Yong Yong Lin, Ya-Chun Chang, Chin-Chou Wang |
| المصدر: | PLoS ONE, Vol 12, Iss 5, p e0176575 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Proteomics, 0301 basic medicine, Pathology, Pulmonology, Microarrays, Apnea, medicine.medical_treatment, Protein Expression, Gene Expression, lcsh:Medicine, Excessive daytime sleepiness, Blood Pressure, Apoptosis, Comorbidity, Severity of Illness Index, Vascular Medicine, Gastroenterology, 0302 clinical medicine, Chronic Kidney Disease, Medicine and Health Sciences, Gene Regulatory Networks, Continuous positive airway pressure, lcsh:Science, Sleep Apnea, Obstructive, Multidisciplinary, Cell Death, Sleep apnea, Intermittent hypoxia, Middle Aged, Bioassays and Physiological Analysis, Neurology, Nephrology, Cell Processes, Hypertension, Female, Junctional Complexes, medicine.symptom, Signal Transduction, Research Article, Adult, Cell Physiology, medicine.medical_specialty, Sleep Apnea, Disorders of Excessive Somnolence, Research and Analysis Methods, Tight Junctions, 03 medical and health sciences, Internal medicine, Severity of illness, Gene Expression and Vector Techniques, Genetics, medicine, Humans, Genetic Predisposition to Disease, Molecular Biology Techniques, Molecular Biology, Molecular Biology Assays and Analysis Techniques, business.industry, Gene Expression Profiling, lcsh:R, Biology and Life Sciences, Cell Biology, medicine.disease, nervous system diseases, respiratory tract diseases, Obstructive sleep apnea, Gene expression profiling, 030104 developmental biology, Gene Expression Regulation, Leukocytes, Mononuclear, lcsh:Q, Sleep Disorders, business, Biomarkers, 030217 neurology & neurosurgery, Genome-Wide Association Study, Kidney disease |
| الوصف: | We aimed to identify novel molecular associations between chronic intermittent hypoxia with re-oxygenation and adverse consequences in obstructive sleep apnea (OSA). We analyzed gene expression profiles of peripheral blood mononuclear cells from 48 patients with sleep-disordered breathing stratified into four groups: primary snoring (PS), moderate to severe OSA (MSO), very severe OSA (VSO), and very severe OSA patients on long-term continuous positive airway pressure treatment (VSOC). Comparisons of the microarray gene expression data identified eight genes up-regulated with OSA and down-regulated with CPAP treatment, and five genes down-regulated with OSA and up-regulated with CPAP treatment. Protein expression levels of two genes related to endothelial tight junction (AMOT P130, and PLEKHH3), and three genes related to anti-or pro-apoptosis (BIRC3, ADAR1 P150, and LGALS3) were all increased in the VSO group, while AMOT P130 was further increased, and PLEKHH3, BIRC3, and ADAR1 P150 were all decreased in the VSOC group. Subgroup analyses revealed that AMOT P130 protein expression was increased in OSA patients with excessive daytime sleepiness, BIRC3 protein expression was decreased in OSA patients with hypertension, and LGALS3 protein expression was increased in OSA patients with chronic kidney disease. In vitro short-term intermittent hypoxia with re-oxygenation experiment showed immediate over-expression of ADAR1 P150. In conclusion, we identified a novel association between AMOT/PLEKHH3/BIRC3/ADAR1/LGALS3 over-expressions and high severity index in OSA patients. AMOT and GALIG may constitute an important determinant for the development of hypersomnia and kidney injury, respectively, while BIRC3 may play a protective role in the development of hypertension. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f38fe541128cc4bc2be6e62580357aeTest https://doi.org/10.1371/journal.pone.0176575Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0f38fe541128cc4bc2be6e62580357ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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