Mitochondrial Transport Processes and Oxidation of NADH by Hypotonically-Treated Boar Spermatozoa
| العنوان: | Mitochondrial Transport Processes and Oxidation of NADH by Hypotonically-Treated Boar Spermatozoa |
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| المؤلفون: | P. K. Tubbs, J Calvin |
| المصدر: | European Journal of Biochemistry. 89:315-320 |
| بيانات النشر: | Wiley, 1978. |
| سنة النشر: | 1978 |
| مصطلحات موضوعية: | Male, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Swine, Uncoupling Agents, Pyruvate transport, Malate-aspartate shuttle, Mitochondrion, Biology, Biochemistry, Malate dehydrogenase, Tricarboxylate, chemistry.chemical_compound, Lactate dehydrogenase, Animals, Dicarboxylic Acids, Mitochondrial transport, Biological Transport, NAD, Spermatozoa, Mitochondria, Kinetics, Hypotonic Solutions, chemistry, Oligomycins, Oxidation-Reduction |
| الوصف: | 1 After hypotonic treatment spermatozoa have metabolic characteristics of mitochondria isolated from other cells. Ejaculated boar spermatozoa treated in this way can oxidise external NADH via both a lactate-pyruvate shuttle and a malate-aspartate cycle; this oxidation is coupled to the phosphorylation of ADP. 2 The dicarboxylate transport inhibitors butylmalonate, phenylsuccinate and bathophenanthroline sulphonate inhibit NADH oxidation dependent on added malate, glutamate and aspartate. α-Cyanocinnamate, a strong inhibitor of pyruvate transport, inhibits lactate-dependent NADH oxidation. 3 NADH oxidation dependent on malate, glutamate and aspartate is inhibited by uncoupling agents, but lactate-dependent NADH oxidation is stimulated. 4 Lactate-dependent NADH oxidation is inhibited by oxamate, an inhibitor of lactate dehydrogenase. Aminooxyacetate, an aminotransferase inhibitor, inhibits glutamate, malate and aspartate-dependent NADH oxidation. 5 Hypotonically-treated spermatozoa retain radioactivity after incubation with l-[U-14C]malate, [1.5-14C]citrate or [2-14C]malonate. Exchanges of retained radioactivity with various substrates indicate that dicarboxylate and tricarboxylate exchange carriers exist in the mitochondrial membrane. |
| تدمد: | 1432-1033 0014-2956 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d0fc1af72c817c396eb396094751a0bTest https://doi.org/10.1111/j.1432-1033.1978.tb20929.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0d0fc1af72c817c396eb396094751a0b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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