Sugars are both nutrients and important signal molecules in higher plants. Sugar transporters (STs) are involved in sugar loading and unloading and facilitate sugar transport across membranes. Tobacco (Nicotiana tabacum) is a model plant and one of the most significant plants economically. In our research, 92 N. tabacum ST (NtST) genes were identified and classified into eight distinct subfamilies in the tobacco genome based on phylogenetic analysis. Exon-intron analysis revealed that each subfamily manifested closely associated gene architectural features based on a comparable number or length of exons. Tandem repetition and purifying selection were the main factors of NtST gene evolution. A search for cis-regulatory elements in the promoter sequences of the NtST gene families suggested that they are probably regulated by light, plant hormones, and abiotic stress factors. We performed a comprehensive expression study in different tissues, viarious abiotic and phytohormone stresses. The results revealed different expression patterns and the functional diversification of NtST genes. The resulting data showed that NtSFP1 was highly expressed all measured five tobacco tissues, and also regulated by the MeJA, and temperature stress. In addition, the virus-induced NibenSFP1 silencing in tobacco and detected dramatically enhanced glucose content, indicating the NtSFP1 might regulate the glucose content and involved in MeJA signaling way to response the temperature stress. In general, our findings provide useful information on understanding the roles of STs in phytohormone signaling way and abiotic stresses in N. tabacum.
