High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue
| العنوان: | High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue |
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| المؤلفون: | Eline N. Kuipers, Vasudev Kantae, Boukje C. Eveleens Maarse, Susan M. van den Berg, Robin van Eenige, Kimberly J. Nahon, Anne Reifel-Miller, Tamer Coskun, Menno P. J. de Winther, Esther Lutgens, Sander Kooijman, Amy C. Harms, Thomas Hankemeier, Mario van der Stelt, Patrick C. N. Rensen, Mariëtte R. Boon |
| المساهمون: | Experimental Immunology, Medical Biochemistry, ACS - Atherosclerosis & ischemic syndromes, AII - Inflammatory diseases |
| المصدر: | Frontiers in physiology, 10(JAN):1913. Frontiers Media S.A. Frontiers in Physiology Frontiers in Physiology, 9, 1913 Frontiers in Physiology, 9 Frontiers in Physiology, Vol 9 (2019) |
| بيانات النشر: | Frontiers Media SA, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | medicine.medical_specialty, Calorie, diet-induced obesity, Physiology, Adipose tissue, White adipose tissue, NAPE-PLD, lcsh:Physiology, chemistry.chemical_compound, Downregulation and upregulation, white adipose tissue, Internal medicine, Physiology (medical), Brown adipose tissue, medicine, endocannabinoids, Original Research, lcsh:QP1-981, Chemistry, brown adipose tissue, Anandamide, medicine.disease, Obesity, Endocannabinoid system, medicine.anatomical_structure, Endocrinology, lipids (amino acids, peptides, and proteins) |
| الوصف: | The endocannabinoid system (ECS) controls energy balance by regulating both energy intake and energy expenditure. Endocannabinoid levels are elevated in obesity suggesting a potential causal relationship. This study aimed to elucidate the rate of dysregulation of the ECS, and the metabolic organs involved, in diet-induced obesity. Eight groups of age-matched male C57Bl/6J mice were randomized to receive a chow diet (control) or receive a high fat diet (HFD, 45% of calories derived from fat) ranging from 1 day up to 18 weeks before euthanasia. Plasma levels of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (N-arachidonoylethanolamine, AEA), and related N-acylethanolamines, were quantified by UPLC-MS/MS and gene expression of components of the ECS was determined in liver, muscle, white adipose tissue (WAT) and brown adipose tissue (BAT) during the course of diet-induced obesity development. HFD feeding gradually increased 2-AG (+132% within 4 weeks, P < 0.05), accompanied by upregulated expression of its synthesizing enzymes Daglα and β in WAT and BAT. HFD also rapidly increased AEA (+81% within 1 week, P < 0.01), accompanied by increased expression of its synthesizing enzyme Nape-pld, specifically in BAT. Interestingly, Nape-pld expression in BAT correlated with plasma AEA levels (R2 = 0.171, β = 0.276, P < 0.001). We conclude that a HFD rapidly activates adipose tissue depots to increase the synthesis pathways of endocannabinoids that may aggravate the development of HFD-induced obesity.. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-042X |
| DOI: | 10.3389/fphys.2018.01913 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cbfd74f64ce4acd3f685d7867f02c4cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0cbfd74f64ce4acd3f685d7867f02c4c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664042X |
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| DOI: | 10.3389/fphys.2018.01913 |