The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis
| العنوان: | The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis |
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| المؤلفون: | Justine S. Gibson, Philip F. Markham, Nadeeka K. Wawegama, Megan E. Vance, Jose Perez-Casal, M.L. Schibrowski, Marc S. Marenda, Anna Kanci, Timothy J. Mahony, Glenn F. Browning, Peter Mansell, Tamsin S. Barnes |
| المصدر: | Veterinary Sciences, Vol 5, Iss 1, p 27 (2018) Veterinary Sciences Veterinary Sciences; Volume 5; Issue 1; Pages: 27 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Mycoplasma bovis, Veterinary medicine, 040301 veterinary sciences, 030106 microbiology, serology, specificity, medicine.disease_cause, Article, Serology, Western blotting, 0403 veterinary science, 03 medical and health sciences, Immune system, medicine, Seroconversion, lcsh:Veterinary medicine, General Veterinary, biology, business.industry, 04 agricultural and veterinary sciences, sensitivity, biology.protein, lcsh:SF600-1100, ELISA, Antibody, business |
| الوصف: | Mycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the outcomes of studies that use these assays. Therefore, the current study used bovine sera sourced from M. bovis exposure studies from three countries to estimate the Se and Sp of two commercial M. bovis enzyme-linked immunosorbent assays (ELISA), BIO K302 and BIO K260, and Western blotting. Western blotting had the highest Se estimate of 74% (95% confidence interval (CI): 16–98%), compared to the BIO K302: 47% (95% CI: 10–87%) and BIO K260: 28% (95% CI: 1–92%). However, for Sp, the BIO K302: 96% (95% CI: 87–99%) and the BIO K260: 100% (95% CI: 93–100%) out-performed Western blotting: 88% (95% CI: 56–98%). Western blotting was the best assay for detecting seroconversion, correctly identifying 61% (95% CI: 29–86%) of exposed animals compared to 35% for BIO K302 (95% CI: 21–54%) and 8% for BIO K260 (95% CI: 0–87%). While none of the methods assessed had high Se and Sp, the availability of these estimates will aid in the interpretation of studies that use these assays. The results of this study highlight the difficulties encountered when using serology to detect exposure to M. bovis in cattle. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2306-7381 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0aaa3240c7f751fb04a44fa5503f5ea6Test http://www.mdpi.com/2306-7381/5/1/27Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0aaa3240c7f751fb04a44fa5503f5ea6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23067381 |
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