Differences in gluten protein composition between old and modern durum wheat genotypes in relation to 20th century breeding in Italy
| العنوان: | Differences in gluten protein composition between old and modern durum wheat genotypes in relation to 20th century breeding in Italy |
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| المؤلفون: | Michele Andrea De Santis, Peter R. Shewry, Zina Flagella, Pasquale De Vita, Luigia Giuzio, Marcella Michela Giuliani, Alison Lovegrove |
| المصدر: | European Journal of Agronomy |
| بيانات النشر: | Elsevier, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, durum wheat old and modern genotypes, Soil Science, Introgression, Plant Science, ω-gliadin, 01 natural sciences, Article, Coeliac disease, 0404 agricultural biotechnology, Glutenin, Genotype, medicine, Allele, ComputingMethodologies_COMPUTERGRAPHICS, chemistry.chemical_classification, biology, nutritional and metabolic diseases, food and beverages, 04 agricultural and veterinary sciences, medicine.disease, 040401 food science, Gluten, Wheat allergy, Sds-page, wheat allergy, sds-page, chemistry, Agronomy, gluten, biology.protein, Gliadin, Agronomy and Crop Science, Durum wheat old and modern genotypes, 010606 plant biology & botany |
| الوصف: | Graphical abstract Highlights • Higher B-type LMW-GS expression in modern genotypes contributes to gluten strength. • A marked decrease in ω-type gliadin expression is observed in modern genotypes. • Differences in ω-5 gliadin expression appear also related to the number of isoforms. • Old and modern genotypes show similar α- and γ-type gliadin expression. • A higher ω-gliadins and HMW-GS content was observed under water deficit. The impact of breeding on grain yields of wheat varieties released during the 20th century has been extensively studied, whereas less information is available on the changes in gluten quality associated with effects on the amount and composition of glutenins and gliadins. In order to explore the effects of breeding during the 20th century on gluten quality of durum wheat for processing and health we have compared a set of old and modern Italian genotypes grown under Mediterranean conditions. The better technological performance observed for the modern varieties was found to be due not only to the introgression of superior alleles of high (HMW-GS) and low molecular weight (LMW-GS) glutenin subunits encoded at Glu-B1 and Glu-B3 loci, but also to differential expression of specific storage proteins. In particular, the higher gluten index observed in modern genotypes was correlated with an increased glutenin/gliadin ratio and the expression of B-type LMW-GS which was, on average, two times higher in the modern than in the old group of durum wheat genotypes. By contrast, no significant differences were found between old and modern durum wheat genotypes in relation to the expression of α-type and γ-type gliadins which are major fractions that trigger coeliac disease (CD) in susceptible individuals. Furthermore, a drastic decrease was observed in the expression of ω-type gliadins in the modern genotypes, mainly ω-5 gliadin (also known as Tri a 19) which is a major allergen in wheat dependent exercise induced anaphylaxis (WDEIA). Immunological and 2DE SDS-PAGE analyses indicated that these differences could be related either to a general down-regulation or to differences in numbers of isoforms. Lower rainfall during grain filling period was related to overall higher expression of HMW-GS and ω-gliadins. In conclusion, breeding activity carried out in Italy during the 20th century appears to have improved durum wheat gluten quality, both in relation to technological performance and allergenic potential. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::096e1e92a380edba206c98466ce11559Test https://repository.rothamsted.ac.uk/download/56fb215dac6180470218c8ef96a9adb12543d512ac14d28b92d7b5b3cd110cb0/4394463/1-s2.0-S116103011730045X-main.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....096e1e92a380edba206c98466ce11559 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |