Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes
| العنوان: | Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes |
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| المؤلفون: | Qin Liu, Pei He, Muxiao Li, Jiaying Guo, Yali Sun, Yangnan Zhao, Zheng Nie, Ao-Yang Siqi, Xueyan Zhan, Cuiqin Huang, Junlong Zhao, Xiaoying Luo, Sen Wang, Long Yu, Lan He |
| المصدر: | Parasites & Vectors Parasites & Vectors, Vol 11, Iss 1, Pp 1-11 (2018) |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Models, Molecular, 0301 basic medicine, Erythrocytes, Buffaloes, Protein Conformation, Cellular localization, 030231 tropical medicine, Protozoan Proteins, Babesia, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Immunoreactivity, 0302 clinical medicine, Babesia orientalis, Babesiosis, Complementary DNA, Organelle, Animals, lcsh:RC109-216, Cloning, Molecular, Phylogeny, Apical organelle complex, Babesia bigemina, biology, Molecular mass, Research, Native form, Computational Biology, Spherical body, Babesia bovis, biology.organism_classification, Fusion protein, Molecular biology, Protein Transport, 030104 developmental biology, Infectious Diseases, Parasitology, Genome, Protozoan |
| الوصف: | Background The spherical body, a membrane bound organelle localized in the apical organelle complex, is unique to Babesia and Theileria spp. The spherical body proteins (SBPs) secreted by spherical bodies include SBP1, SBP2, SBP3 and SBP4. Up to now, only SBP3 has been characterized in Babesia orientalis. Methods The BoSBP4 gene was amplified from cDNA and gDNA and cloned into the pGEX-6P-1 vector by homologous recombination, sequenced and analyzed by bioinformatics tools. The amino acid (aa) sequence of BoSBP4 was compared with that of Babesia bovis and Babesia bigemina as well as SBP3 of B. orientalis. The immunoreactivity was evaluated by incubating recombinant BoSBP4 (rBoSBP4) with the serum of B. orientalis-infected water buffalo. The native form of BoSBP4 was identified by incubating lysate of B. orientalis-infected water buffalo erythrocytes with the anti-rBoSBP4 mouse serum. The cellular localization of BoSBP4 was determined by indirect immunofluorescence assay. Results The full length of the BoSBP4 gene was estimated to be 945 bp without introns, encoding a 314 aa polypeptide with a predicted molecular weight of 37 kDa. The truncated recombinant protein was expressed from 70 to 945 bp as a GST fusion protein with a practical molecular weight of 70 kDa. BoSBP4 shared a 40% and 30% identity with B. bovis and B. bigemina, respectively. Furthermore, it was 31% identical to SBP3 of B. orientalis. BoSBP4 was identified in the lysate of B. orientalis-infected water buffalo erythrocytes with a molecular weight of 37 kDa, corresponding to the expected molecular mass of BoSBP4. The result of rBoSBP4 with positive serum revealed that BoSBP4 can elicit an immune response to B. orientalis-infected water buffalo. The cellular localization of BoSBP4 was detected to be adjacent to the merozoite nucleus in the intracellular phase, followed by the diffusion of the fluorescence of BoSBP4 into the cytoplasm of B. orientalis-infected erythrocytes as puncta-like specks and a gradual increase of the fluorescence. Conclusions In this study, SBP4 in B. orientalis was characterized for the first time. It may play a key role in interaction with the host cell by being secreted into the cytoplasm of the B. orientalis-infected erythrocytes to facilitate parasite growth and reproduction. |
| تدمد: | 1756-3305 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::082f51de11c412002edc1f2c81c32eedTest https://doi.org/10.1186/s13071-018-3018-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....082f51de11c412002edc1f2c81c32eed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17563305 |
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