Evaluation of MGP gene expression in colorectal cancer
| العنوان: | Evaluation of MGP gene expression in colorectal cancer |
|---|---|
| المؤلفون: | Daniel M. Tiago, Natércia Conceição, Ana Margarida Vaz, Artur Antunes, Susana Vicente, Paulo Caldeira, Helena Caiado, Horácio Guerreiro, Ana Marreiros, M. Leonor Cancela, Jose Luis Enriquez |
| المصدر: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Colorectal cancer, Angiogenesis, RUNX2, Core Binding Factor Alpha 1 Subunit, Matrix gla protein, 03 medical and health sciences, 0302 clinical medicine, Gene expression, Genetics, medicine, Transcription factors, Humans, Aged, Neoplasm Staging, Aged, 80 and over, Genetics & Heredity, Extracellular Matrix Proteins, biology, Colorectal Cancer (CRC), Calcium-Binding Proteins, Cancer, nutritional and metabolic diseases, General Medicine, Middle Aged, medicine.disease, Prognosis, Survival Analysis, Up-Regulation, Gene Expression Regulation, Neoplastic, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, Keutel syndrome, Cancer research, Immunohistochemistry, Female, Colorectal Neoplasms |
| الوصف: | Purpose: Matrix Gla protein (MGP) is a vitamin K-dependent, gamma-carboxylated protein that was initially found to be a physiological inhibitor of ectopic calcifications affecting mainly cartilage and the vascular system. Mutations in the MGP gene were found to be responsible for a human pathology, the Keutel syndrome, characterized by abnormal calcifications in cartilage, lungs, brain and vascular system. MGP was recently implicated in tumorigenic processes such as angiogenesis and shown to be abnormally regulated in several tumors, including cervical, ovarian, urogenital and breast. This fact has triggered our interest in analyzing the expression of MGP and of its regulator, the transcription factor runt related transcription factor 2 (RUNX2), in colorectal cancer (CRC). Methods: MGP and RUNX2 expression were analyzed in cancer and non-tumor biopsies samples from 33 CRC patients and 9 healthy controls by RT-qPCR. Consequently, statistical analyses were performed to evaluate the clinical-pathological significance of MGP and RUNX2 in CRC. MGP protein was also detected by immunohistochemical analysis. Results: Showed an overall overexpression of MGP in the tumor mucosa of patients at mRNA level when compared to adjacent normal mucosa and healthy control tissues. In addition, analysis of the expression of RUNX2 mRNA demonstrated an overexpression in CRC tissue samples and a positive correlation with MGP expression (Pearson correlation coefficient 0.636; p |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06925cadde85d83a184d4b61b3ad45d3Test https://hdl.handle.net/10400.1/16605Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....06925cadde85d83a184d4b61b3ad45d3 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |