Robust passive and active efflux of cellular cholesterol to a designer functional mimic of high density lipoprotein
| العنوان: | Robust passive and active efflux of cellular cholesterol to a designer functional mimic of high density lipoprotein |
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| المؤلفون: | Michael C. Phillips, Chad A. Mirkin, Daniel J. Rader, Duyen Quach, C. Shad Thaxton, Nicholas N. Lyssenko, Kasey C. Vickers, John S. Millar, Andrea J. Luthi, Kaylin M. McMahon |
| المصدر: | Journal of Lipid Research Journal of Lipid Research, Vol 56, Iss 5, Pp 972-985 (2015) |
| بيانات النشر: | The American Society for Biochemistry and Molecular Biology, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | ATP-binding cassette transporter G1, Cardiotonic Agents, Lipoproteins, Phospholipid, Drug Evaluation, Preclinical, QD415-436, ATP-binding cassette transporter A1, Coronary Artery Disease, 030204 cardiovascular system & hematology, Biology, Biochemistry, Cell Line, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, High-density lipoprotein, Drug Stability, scavenger receptor class B type I, Cricetinae, Animals, Scavenger receptor, Research Articles, Phospholipids, 030304 developmental biology, ATP Binding Cassette Transporter, Subfamily G, Member 1, 0303 health sciences, Apolipoprotein A-I, Cholesterol, Macrophages, Molecular Mimicry, Biological Transport, Cell Biology, Scavenger Receptors, Class B, ATP Binding Cassette Transporter 1, chemistry, ABCG1, ABCA1, biology.protein, Nanoparticles, lipids (amino acids, peptides, and proteins), ATP-Binding Cassette Transporters, Efflux, Gold, atherosclerosis, cholesterol efflux |
| الوصف: | The ability of HDL to support macrophage cholesterol efflux is an integral part of its atheroprotective action. Augmenting this ability, especially when HDL cholesterol efflux capacity from macrophages is poor, represents a promising therapeutic strategy. One approach to enhancing macrophage cholesterol efflux is infusing blood with HDL mimics. Previously, we reported the synthesis of a functional mimic of HDL (fmHDL) that consists of a gold nanoparticle template, a phospholipid bilayer, and apo A-I. In this work, we characterize the ability of fmHDL to support the well-established pathways of cellular cholesterol efflux from model cell lines and primary macrophages. fmHDL received cell cholesterol by unmediated (aqueous) and ABCG1- and scavenger receptor class B type I (SR-BI)-mediated diffusion. Furthermore, the fmHDL holoparticle accepted cholesterol and phospholipid by the ABCA1 pathway. These results demonstrate that fmHDL supports all the cholesterol efflux pathways available to native HDL and thus, represents a promising infusible therapeutic for enhancing macrophage cholesterol efflux. fmHDL accepts cholesterol from cells by all known pathways of cholesterol efflux: unmediated, ABCG1- and SR-BI-mediated diffusion, and through ABCA1. |
| اللغة: | English |
| تدمد: | 1539-7262 0022-2275 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0550abb1901911bc2d06d1f118adbb66Test http://europepmc.org/articles/PMC4409287Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0550abb1901911bc2d06d1f118adbb66 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15397262 00222275 |
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