CIRE1, a novel transcriptionally active Ty1-copia retrotransposon from Citrus sinensis
| العنوان: | CIRE1, a novel transcriptionally active Ty1-copia retrotransposon from Citrus sinensis |
|---|---|
| المؤلفون: | José Antonio Martínez-Izquierdo, Laura Rico-Cabanas |
| المساهمون: | Generalitat de Catalunya, Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Ministerio de Educación y Ciencia (España) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) |
| بيانات النشر: | Springer Nature, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Retroelements, Molecular Sequence Data, GUS reporter system, Retrotransposon, Plant Roots, Genome, chemistry.chemical_compound, Gene Expression Regulation, Plant, Auxin, Transcription (biology), Genetics, Molecular Biology, chemistry.chemical_classification, Methyl jasmonate, Base Sequence, biology, fungi, Terminal Repeat Sequences, food and beverages, General Medicine, Plants, Genetically Modified, biology.organism_classification, Molecular biology, chemistry, Eukaryote, Genome, Plant, Citrus × sinensis, Citrus sinensis |
| الوصف: | LTR retrotransposons (LTR-RTNs) are widespread constituents of eukaryote genomes, particularly plant genomes. Although LTR-RTNs from plants were thought to be transcriptionally silent in somatic tissues, evidences of activity under certain conditions are available for some of them. In order to investigate LTR-RTNs in the Citrus sinensis genome, we analysed them by PCR using degenerate primers corresponding to highly conserved domains. All elements of the two types of LTR-RTN comprise about 23% of the genome, the copia group contribution being higher (13%) than the gypsy one (10%). From dendogram analysis, we report seven new copia RTN families, named CIRE1 to CIRE7. Here, we report on the first complete retrotransposon identified in Citrus (named CIRE1), which has all the features of a typical copia RTN. CIRE1 retrotransposon has around 2,200 full-length copies, contributing to 2.9% of the C. sinensis genome. CIRE1 has a root-specific expression in sweet orange plants. We have also determined that wounding and exogenous application of plant hormones, as methyl jasmonate and auxin, increase the transcription level of CIRE1 in leaf tissues. In addition, we show that CIRE1 5′LTR promoter can drive transient expression of the gus reporter gene in heterologous plant systems. These findings confirm CIRE1 as one of the few transcriptionally active RTNs described in plants and to our knowledge the first one to be reported in Citrus species. LRC was recipient of a predoctoral fellowships from Autonomous Catalan Government (Generalitat) (2001FI 00143). Some funds for this study were provided by CERBA (Generalitat of Catalonia). This work was mainly supported by the grant BIO99-1175 (CICYT) from the MEC of Spanish Govern to JAMI. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05288336381edcb135f70896bf71abc4Test http://hdl.handle.net/10261/248252Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....05288336381edcb135f70896bf71abc4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |