Antioxidant Action of Ellagic Acid Ameliorates Paraquat-Induced A549 Cytotoxicity
| العنوان: | Antioxidant Action of Ellagic Acid Ameliorates Paraquat-Induced A549 Cytotoxicity |
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| المؤلفون: | Tamanna Zerin, Yong-Sik Kim, Ho-Yeon Song |
| المصدر: | Biological and Pharmaceutical Bulletin. 36:609-615 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Paraquat, Antioxidant, Cell Survival, NF-E2-Related Factor 2, medicine.medical_treatment, Pharmaceutical Science, medicine.disease_cause, Antioxidants, Lipid peroxidation, chemistry.chemical_compound, Ellagic Acid, Cell Line, Tumor, NAD(P)H Dehydrogenase (Quinone), medicine, Humans, RNA, Messenger, Viability assay, Pharmacology, chemistry.chemical_classification, A549 cell, Reactive oxygen species, L-Lactate Dehydrogenase, Herbicides, General Medicine, Glutathione, Oxidative Stress, chemistry, Biochemistry, Reactive Oxygen Species, Heme Oxygenase-1, Oxidative stress, Ellagic acid |
| الوصف: | Ellagic acid (EA) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anti-carcinogenesis and anti-oxidation properties. This study aimed to evaluate the effect of EA against paraquat (PQ)-induced oxidative stress. PQ decreased the viability of A549 cells in dose- and time-dependent manners, which was associated with the massive generation of reactive oxygen species (ROS). However, cell viability was significantly recovered by the treatment of EA, from 47.01±1.59% to 66.04±2.84%. The release of lactate dehydrogenase (LDH) was also decreased with the treatment of EA in PQ-treated A549 cells. EA induced the level of expression and activation of nuclear factor-erythroid 2-related factor (Nrf2) and its target cytoprotective and antioxidant genes, heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (NQO1). The antioxidant potential of EA might be directly correlated with the increased expression of HO-1 and NQO1, whose expression may have surmounted the oxidative stress generated by PQ. Notably, EA treatment significantly reduced the levels of biochemical markers as lipid peroxidation, reduced the intracellular ROS level, and surmounted total glutathione level in A549 cells. Data indicate that the antioxidant and cytoprotective properties of EA reduce PQ-induced cytotoxicity in human alveolar A549 cells. |
| تدمد: | 1347-5215 0918-6158 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::049cfa54e804e7b27997f51035eec80fTest https://doi.org/10.1248/bpb.b12-00990Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....049cfa54e804e7b27997f51035eec80f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
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