Characterization of OCT3/4, Nestin, NANOG, CD44 and CD24 as stem cell markers in canine prostate cancer
| العنوان: | Characterization of OCT3/4, Nestin, NANOG, CD44 and CD24 as stem cell markers in canine prostate cancer |
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| المؤلفون: | Priscila Emiko Kobayashi, Renée Laufer Amorim, Andre Augusto Justo, Carlos Eduardo Fonseca-Alves, Camila Dorotea Costa, Michelle M. Story, Chiara Palmieri |
| المساهمون: | Universidade Estadual Paulista (Unesp), The University of Queensland |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Homeobox protein NANOG, Male, Stem cell marker, Biochemistry, Nestin, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, Dogs, Cancer stem cell, Cell Line, Tumor, medicine, Biomarkers, Tumor, Dog, Animals, Cell Self Renewal, CD44, OCT3/4, biology, Cancer, CD24 Antigen, Prostatic Neoplasms, Cell Biology, Nanog Homeobox Protein, medicine.disease, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Hyaluronan Receptors, Phenotype, NANOG, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, biology.protein, Neoplastic Stem Cells, Octamer Transcription Factor-3 |
| الوصف: | Made available in DSpace on 2019-10-06T16:13:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The cancer cell population is heterogeneous, and cancer stem cells (CSCs) are important for tumor growth and maintenance. The CSC population is associated with different neoplastic characteristics, such as cell migration, resistance to apoptosis, radiation therapy and chemotherapy. To increase the knowledge of CSCs in canine prostate cancer (PC), we characterized CSC markers in canine PC tissues and tumorspheres. We performed immunohistochemistry of OCT3/4, Nestin, NANOG, CD44 and CD24 in 10 normal canine prostatic tissue samples, 10 prostatic hyperplastic (PH) tissue samples and 28 PC tissue samples. Then, we established two canine prostate cancer cell cultures and characterized the CSC profile of tumorspheres grown from these cultures. Normal and PH tissues were positive for Nestin, NANOG, CD44 and CD24 only in the basal cell layer. OCT3/4 was expressed in the luminal cells of normal and PH tissues. There was no significant difference in Nestin expression among the prostatic tissues. However, we found higher expression of NANOG and CD44 in canine PC tissues than that in normal and PH tissues. Tumorspheres from canine prostate cancer cells express OCT3/4, Nestin, NANOG and CD44, indicating that these markers may be potential cancer stem cell markers in canine PC. The results obtained can be useful to better characterize the stem cell population in canine prostatic cancer and to guide future studies in comparative oncology. São Paulo State University – UNESP Department of Veterinary Surgery and Anaesthesiology School of Veterinary Medicine and Animal Science São Paulo State University – UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science School of Veterinary Science The University of Queensland, Gatton Campus São Paulo State University – UNESP Department of Veterinary Surgery and Anaesthesiology School of Veterinary Medicine and Animal Science São Paulo State University – UNESP Department of Veterinary Clinic School of Veterinary Medicine and Animal Science FAPESP: 2015/25400-7 FAPESP: 2017/25822-4 |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::048078fc15fdc2ee68af6adad2a968fdTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....048078fc15fdc2ee68af6adad2a968fd |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |