Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain
| العنوان: | Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain |
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| المؤلفون: | Jiyong Meng, Chanjuan Xu, Pierre-André Lafon, Salomé Roux, Michaël Mathieu, Rui Zhou, Pauline Scholler, Emilie Blanc, Jérôme A. J. Becker, Julie Le Merrer, Javier González-Maeso, Patrick Chames, Jianfeng Liu, Jean-Philippe Pin, Philippe Rondard |
| المساهمون: | Huazhong University of Science and Technology [Wuhan] (HUST), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Virginia Commonwealth University (VCU), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Guerineau, Nathalie C., ANR-20-CE18-0011,Nano4Schizo,Les nanobodies des récepteurs mGlu comme agents pharmacologiques innovants pour le traitement des symptômes de la schizophrénie(2020), ANR-20-CE44-0006,SchizoGlu,Signalisation du Récepteur mGlu2 dans le Cerveau: Altérations dans les modèles précliniques de Schizophrénie et Interférences entre Antipsychotiques(2020) |
| المصدر: | Nature Chemical Biology Nature Chemical Biology, 2022, 18, pp.894-903. ⟨10.1038/s41589-022-01050-2⟩ |
| بيانات النشر: | HAL CCSD, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Fluorescence Resonance Energy Transfer, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Brain, Glutamic Acid, Cell Biology, Receptors, Metabotropic Glutamate, Molecular Biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology |
| الوصف: | International audience; Membrane proteins, including ion channels, receptors and transporters, are often composed of multiple subunits and can form large complexes. Their specific composition in native tissues is difficult to determine and remains largely unknown. In this study, we developed a method for determining the subunit composition of endogenous cell surface protein complexes from isolated native tissues. Our method relies on nanobody-based sensors, which enable proximity detection between subunits in time-resolved Förster resonance energy transfer (FRET) measurements. Additionally, given conformation-specific nanobodies, the activation of these complexes can be recorded in native brain tissue. Applied to the metabotropic glutamate receptors in different brain regions, this approach revealed the clear existence of functional metabotropic glutamate (mGlu)2-mGlu4 heterodimers in addition to mGlu2 and mGlu4 homodimers. Strikingly, the mGlu4 subunits appear to be mainly heterodimers in the brain. Overall, these versatile biosensors can determine the presence and activity of endogenous membrane proteins in native tissues with high fidelity and convenience. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1552-4450 1552-4469 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::047b294d11390a4ebb7d45c816ec59d6Test https://hal.science/hal-03699580/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....047b294d11390a4ebb7d45c816ec59d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15524450 15524469 |
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