SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway

التفاصيل البيبلوغرافية
العنوان: SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway
المؤلفون: Qingcheng Yang, Shumin Zhou, Lei Hou, Bin Zhu, Dong-dong Cheng, Tao Ying
المصدر: Oncology Reports
بيانات النشر: Spandidos Publications, 2017.
سنة النشر: 2017
مصطلحات موضوعية: Adult, Male, 0301 basic medicine, CD98hc, Cancer Research, Adolescent, Fusion Regulatory Protein 1, Heavy Chain, SLC3A2, proliferation, Cell, Bone Neoplasms, Biology, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, medicine, Humans, PI3K/AKT/mTOR pathway, Cell Proliferation, Osteosarcoma, Oncogene, Cell growth, Akt/PKB signaling pathway, Articles, Cell Cycle Checkpoints, General Medicine, Cell cycle, medicine.disease, Up-Regulation, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Oncology, Gene Knockdown Techniques, 030220 oncology & carcinogenesis, Cancer research, Female, Signal transduction, Proto-Oncogene Proteins c-akt, Signal Transduction
الوصف: Growing evidence indicates that SLC3A2 (solute carrier family 3 member 2) is upregulated and correlates with tumor growth in multiple types of cancers, while the role of SLC3A2 in human osteosarcoma (OS) is rarely discussed. Thus, the aim of the present study was to demonstrate the expression of SLC3A2 in human osteosarcoma and reveal its biological function and the underlying mechanisms. RT-PCR, western blot analysis and immunohistochemistry (IHC) were used to assess the expression of SLC3A2 in OS samples and cell lines. Cell cycle, Cell Counting Kit-8 (CCK-8) and colony formation assays were used to test the cell survival capacity. To investigate the potential mechanism by which SLC3A2 regulates OS growth, we used a slide-based antibody array. We demonstrated that SLC3A2 was upregulated in OS cell lines as well as OS tissues. High expression of SLC3A2 was correlated with clinical stage and tumor size in OS. Reduced expression of SLC3A2 inhibited OS cell proliferation through G2/M phase arrest. Most importantly, we found that SLC3A2 may regulate OS growth through the PI3K/Akt signaling pathway. In conclusion, SLC3A2 is upregulated in OS and plays a crucial role in tumor growth. Targeting SLC3A2 may provide a new therapeutic strategy for OS.
تدمد: 1791-2431
1021-335X
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04507e502c235f86d6a0b5b0318f5107Test
https://doi.org/10.3892/or.2017.5530Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....04507e502c235f86d6a0b5b0318f5107
قاعدة البيانات: OpenAIRE