Antioxidant Capacity of Thistle (Cirsium japonicum) in Various Drying Methods and their Protection Effect on Neuronal PC12 Cells and Caenorhabditis elegans
العنوان: | Antioxidant Capacity of Thistle (Cirsium japonicum) in Various Drying Methods and their Protection Effect on Neuronal PC12 Cells and Caenorhabditis elegans |
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المؤلفون: | Miran Jang, Gun Hee Kim, Kee-Hong Kim |
المصدر: | Antioxidants Volume 9 Issue 3 Antioxidants, Vol 9, Iss 3, p 200 (2020) Department of Food Science Faculty Publications |
بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | 0301 basic medicine, antioxidant, Antioxidant, food.ingredient, Physiology, medicine.medical_treatment, Clinical Biochemistry, phenolic compounds, thistle (Cirsium japonicum), reactive oxygen species (ROS), PC 12 cells, Caenorhabditis elegans, Biochemistry, Article, 03 medical and health sciences, chemistry.chemical_compound, food, Chlorogenic acid, Lactate dehydrogenase, medicine, Food science, Viability assay, Molecular Biology, thistle (Cirsium japonicum), phenolic compounds, antioxidant, reactive oxygen species (ROS), PC 12 cells, Caenorhabditis elegans, chemistry.chemical_classification, Reactive oxygen species, 030109 nutrition & dietetics, biology, Chemistry, lcsh:RM1-950, Pectolinarin, Cell Biology, biology.organism_classification, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, Thistle |
الوصف: | The aim of this study was, firstly, to evaluate the phenol profile of thistle (Cirsium japonicum, CJ) by High performance liquid chromatography-electrospray ionization&ndash mass spectrometry (HPLC&ndash ESI&ndash MS), dried by different methods (90 ° C hot-air, 70 ° C hot-air, shade-, and freeze-drying). Secondly, we aimed to evaluate the relationship between phenolic compounds content and antioxidant properties. CJ contained chlorogenic acid, linarin, and pectolinarin. Total phenolic contents of CJ significantly decreased under hot-air-drying condition, especially chlorogenic acid contents in CJ have been reduced by 85% and 60% for 90 ° C and 70 ° C hot-air-drying, respectively. We evaluated the protective effect on adrenal pheochromocytoma (PC12) cells and Caenorhabditis elegans using shade-dried CJ, which has the largest phenolic contents and the strongest antioxidant property. CJ-treated PC 12 cells dose-dependently exhibited the protective effects against reactive oxygen species (ROS), while cell viability increases, lactate dehydrogenase release decreases, and ROS formation decreases. Furthermore, CJ has also shown protection against ROS in C. elegans. Consequently, CJ contributed to lifespan extension under ROS stress without influencing the physiological growth. |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 2076-3921 |
DOI: | 10.3390/antiox9030200 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02765ef424764286249d6fdd31b100e0Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....02765ef424764286249d6fdd31b100e0 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 20763921 |
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DOI: | 10.3390/antiox9030200 |