Genetic and Morphologic Identification of Spirometra ranarum in Myanmar
| العنوان: | Genetic and Morphologic Identification of Spirometra ranarum in Myanmar |
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| المؤلفون: | Seongjun Choe, Hansol Park, Woon-Mok Sohn, Dongmin Lee, Keeseon S. Eom, Jong-Yil Chai, Hyeong-Kyu Jeon, Mohammed Mebarek Bia, Sung-Jong Hong, Yeseul Kang, Sang-Hwa Lee |
| المصدر: | The Korean Journal of Parasitology |
| بيانات النشر: | Korean Society for Parasitology, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, 030231 tropical medicine, Zoology, Myanmar, Biology, nad1, 03 medical and health sciences, Dogs, 0302 clinical medicine, Hoplobatrachus, Phylogenetics, Plerocercoid, Animals, Spirometra, Phylogeny, Morphometrics, Phylogenetic tree, Helminth Proteins, Sequence Analysis, DNA, DNA, Helminth, spargana, 030108 mycology & parasitology, biology.organism_classification, Spirometra ranarum, cox1, Molecular analysis, molecular detection, Infectious Diseases, GenBank, Original Article, Parasitology, Anura |
| الوصف: | In the present study, we identified a Spirometra species of Myanmar origin (plerocercoid) by molecular analysis using mitochondrial cox1 and nad1 genes, as well as by morphological observations of an adult tapeworm. Spargana specimens were collected from a paddy-field in Taik Kyi Township Tarkwa Village, Yangon, Myanmar in December 2017. A total of 5 spargana were obtained from 20 frogs Hoplobatrachus rugulosus; syn: Rana rugulosa (Wiegmann, 1834) or R. tigrina (Steindachner, 1867). The plerocercoids were used for experimental infection of a dog. After 4 weeks of infection, an adult tapeworm was recovered from the intestine of the dog. Morphologically, the distinct features of Spirometra sp. (Myanmar origin) relative to S. erinaceieuropaei and S. decipiens include a uterine morphology comprising posterior uterine coils that larger than the terminal uterine ball and coiling of the uteri diagonally (swirling) rather than spirally. The cox1 sequences (1,566 bp) of the Myanmar-origin Spirometra species showed 97.9% similarity to a reference sequence of S. decipiens (GenBank no. {"type":"entrez-nucleotide","attrs":{"text":"KJ599679","term_id":"646228151","term_text":"KJ599679"}}KJ599679) and 90.5% similarity to a reference sequence of S. erinaceieuropaei (GenBank no. {"type":"entrez-nucleotide","attrs":{"text":"KJ599680","term_id":"646228164","term_text":"KJ599680"}}KJ599680). Phylogenetic tree topologies were identical and presented high confidence level of values for the 3 major branches of the 3 Spirometra species in cox1 and nad1 genes. These results indicated that Myanmar-origin Spirometra species coincided with those of S. ranarum and may be considered as a valid species. |
| تدمد: | 1738-0006 0023-4001 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::022e93346cc2981c09eb0575863aa050Test https://doi.org/10.3347/kjp.2018.56.3.275Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....022e93346cc2981c09eb0575863aa050 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17380006 00234001 |
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