Studies of the binding of diolepoxide metabolites of polycyclic aromatic hydrocarbons to DNA using electrofluorescence polarization spectroscopy
| العنوان: | Studies of the binding of diolepoxide metabolites of polycyclic aromatic hydrocarbons to DNA using electrofluorescence polarization spectroscopy |
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| المؤلفون: | Michael H. Tinker, Martin R. Osborne, Stuart A. Windsor, Albrecht Seidel |
| المصدر: | Carcinogenesis. 17:605-608 |
| بيانات النشر: | Oxford University Press (OUP), 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Bay-Region, Polycyclic Aromatic Hydrocarbon, Chrysene, Cancer Research, Stereochemistry, Guanine, Benzo(c)phenanthrene, Stereoisomerism, General Medicine, Phenanthrenes, Phenanthrene, Adduct, DNA Adducts, chemistry.chemical_compound, Spectrometry, Fluorescence, chemistry, Benzo(a)pyrene, Polycyclic Hydrocarbons, Polycyclic Aromatic Hydrocarbons, DNA |
| الوصف: | In the electrofluorescence method, a solution of DNA with covalently bound polycyclic hydrocarbons is placed in an electric field, and changes in the intensity of polarized fluorescence are observed. Under the correct conditions, these charges can be used to determine a value for the angle psi between the long axis of the hydrocarbon molecule and the axis of the DNA helix. For DNA or poly(dA-dT) treated with each stereoisomer of anti-benzo[c]phenanthrene diolepoxide, psi ranged from 55 degrees to 61 degrees, consistent with a mixture of quasi-intercalated adenine adducts and externally bound guanine adducts. Similar results were obtained with another set of 'fjord-region' diolepoxides, derived from benzo[c]chrysene. Adducts in DNA treated with diolepoxides derived from chrysene, 5-methylchrysene or 6-methylchrysene gave psi of about 53 degrees, so the predominant adducts are externally bound, probably in the minor groove of DNA. |
| تدمد: | 1460-2180 0143-3334 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02173e94f2d5be5e6d94a87e1ea766a1Test https://doi.org/10.1093/carcin/17.3.605Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....02173e94f2d5be5e6d94a87e1ea766a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602180 01433334 |
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