Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury
| العنوان: | Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury |
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| المؤلفون: | Stephan W. Glasser, Peter D. Sly, Jeffrey A. Whitsett, Timothy E. Weaver, Gary F. Ross, Machiko Ikegami, Juliana J. Conkright |
| المصدر: | Journal of Applied Physiology. 92:519-526 |
| بيانات النشر: | American Physiological Society, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Physiology, Ratón, Proteolipids, Mice, Transgenic, Hyperoxia, Lung injury, Biology, medicine.disease_cause, Mice, chemistry.chemical_compound, Pulmonary surfactant, In vivo, Physiology (medical), Phosphatidylcholine, Pressure, medicine, Animals, Surface Tension, Lung, Mutation, Proteins, Pulmonary Surfactants, Pneumonia, Molecular biology, Oxygen, Pulmonary Alveoli, Mechanism of action, chemistry, Immunology, Phosphatidylcholines, Respiratory Mechanics, Cytokines, medicine.symptom, Lung Volume Measurements, Bronchoalveolar Lavage Fluid, Protein C, medicine.drug |
| الوصف: | Although the surface properties of surfactant protein (SP)-B and SP-C are similar, the contributions that either protein may make to lung function have not been identified in vivo. Mutations in SP-B cause lethal respiratory failure at birth; however, SP-B null mice are deficient in both SP-B and SP-C. To identify potential contributions of SP-C to lung function in vivo, the following transgenic mice were generated and exposed to 95% O2for 3 days: (SP-B+/+,SP-C+/+), (SP-B+/+, SP-C−/−), (SP-B+/−,SP-C+/+), (SP-B+/−,SP-C+/−), and (SP-B+/−,SP-C−/−). Hyperoxia altered pressure-volume curves in mice that were heterozygous for SP-B, and these values were further decreased in (SP-B+/−,SP-C−/−) mice. Likewise, alveolar interleukin (IL)-6 and IL-1β were maximally increased by O2exposure of (SP-B+/−,SP-C−/−) mice compared with the other genotypes. Lung hysteresivity was lower in the (SP-B+/−,SP-C−/−) mice. Surfactant isolated from (SP-B+/+,SP-C−/−) and (SP-B+/−,SP-C−/−) mice failed to stabilize the surface tension of microbubbles, showing that SP-C plays a role in stabilization or recruitment of phospholipid films at low bubble radius. Genetically decreased levels of SP-B combined with superimposed O2-induced injury reveals the distinct contribution of SP-C to pulmonary function in vivo. |
| تدمد: | 1522-1601 8750-7587 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::020e71ab397eb1b9e3ce1d3ff46221fbTest https://doi.org/10.1152/japplphysiol.00459.2001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....020e71ab397eb1b9e3ce1d3ff46221fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221601 87507587 |
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