Effects of fermented Aphanizomenon flos-aquae on the caecal microbiome of mice fed a high-sucrose and low-dietary fibre diet
| العنوان: | Effects of fermented Aphanizomenon flos-aquae on the caecal microbiome of mice fed a high-sucrose and low-dietary fibre diet |
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| المؤلفون: | Hajime Takahashi, Bon Kimura, Takashi Kuda, Moemi Takei, Miyu Taniguchi |
| المصدر: | Journal of Applied Phycology. 33:397-407 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, biology, 010604 marine biology & hydrobiology, Lactococcus lactis, Aphanizomenon flos-aquae (dietary supplement), food and beverages, Plant Science, Aquatic Science, biology.organism_classification, Aphanizomenon, 01 natural sciences, parasitic diseases, Food science, Microbiome, Alistipes, Lactobacillus plantarum, Bacteria, 010606 plant biology & botany, Ruminococcaceae |
| الوصف: | Aphanizomenon flos-aquae, a freshwater cyanobacterium harvested from Upper Klamath Lake, Oregon, USA, is employed as a functional food material. Aphanizomenon flos-aquae can be fermented with Lactobacillus plantarum and Lactococcus lactis subsp. lactis. Here, to determine the dietary effects of A. flos-aquae (AFA) and fermented A. flos-aquae (F-AFA) on the mouse gut microbiome, high-sucrose diet containing no dietary fibre (NF), 5% AFA or 5% F-AFA was administered to ICR mice for 14 days. Compared with those in mice fed the NF, lower body weight gains and epididymal fat pad tissue weights were observed in mice fed the diet containing F-AFA. After feeding, the caecal microbiome was analysed by 16S rRNA (V4) gene amplicon sequencing using the Illumina MiSeq system. Sequences were clustered into operational taxonomic units, with a 97% identity cutoff, using the QIIME2 workflow script and SILVA database. The caecal microbiome alpha diversity was high in mice fed the F-AFA diet. The abundances of Muribaculum- and Alistipes inops-like bacteria belonging to the phylum Bacteroidetes and Ruminococcaceae UCG-014-like bacteria belonging to phylum Firmicutes were higher in the F-AFA group than in the AFA group. Isolation of typical F-AFA-susceptible gut indigenous bacteria and functional studies of the isolates in the presence of F-AFA are warranted to validate the current findings. |
| تدمد: | 1573-5176 0921-8971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ffb3c88db8a1b77a0e6d145197f26233Test https://doi.org/10.1007/s10811-020-02306-xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........ffb3c88db8a1b77a0e6d145197f26233 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735176 09218971 |
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