Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding
| العنوان: | Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding |
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| المؤلفون: | Fu-Jia Tian, Chen Zhang, Erchi Zhou, Hai-Long Dong, Zhi-Jie Tan, Xing-Hua Zhang, Liang Dai |
| المصدر: | Proceedings of the National Academy of Sciences. 120 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | Nucleic acid deformations play important roles in many biological processes. The physical understanding of nucleic acid deformation by environmental stimuli is limited due to the challenge in the precise measurement of RNA and DNA deformations and the complexity of interactions in RNA and DNA. Magnetic tweezers experiments provide an excellent opportunity to precisely measure DNA and RNA twist changes induced by environmental stimuli. In this work, we applied magnetic tweezers to measure double-stranded RNA twist changes induced by salt and temperature changes. We observed RNA unwinds when lowering salt concentration, or increasing temperature. Our molecular dynamics simulations revealed the mechanism: lowering salt concentration or increasing temperature enlarges RNA major groove width, which causes twist decrease through twist-groove coupling. Combining these results with previous results, we found some universality in RNA and DNA deformations induced by three different stimuli: salt change, temperature, and stretching force. For RNA, these stimuli first modify the major groove width, which is transduced into twist change through twist-groove coupling. For DNA, these stimuli first modify diameter, which is transduced into twist change through twist-diameter coupling. Twist-groove coupling and twist-diameter coupling appear to be utilized by protein binding to reduce DNA and RNA deformation energy cost upon protein binding. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ecff4035bbfce95eadd1a5a6d6fa0f87Test https://doi.org/10.1073/pnas.2218425120Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ecff4035bbfce95eadd1a5a6d6fa0f87 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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