A Chlamydia-Specific TCR-Transgenic Mouse Demonstrates Th1 Polyfunctionality with Enhanced Effector Function
| العنوان: | A Chlamydia-Specific TCR-Transgenic Mouse Demonstrates Th1 Polyfunctionality with Enhanced Effector Function |
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| المؤلفون: | Taylor B. Poston, McKensie Wall, Catherine M. O'Connell, Ali N. Russell, Yanyan Qu, Toni Darville, Uma M. Nagarajan, Lauren C. Frazer, Jenna Girardi |
| المصدر: | The Journal of Immunology. 199:2845-2854 |
| بيانات النشر: | The American Association of Immunologists, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Adoptive cell transfer, Cellular immunity, biology, Effector, T cell, Immunology, medicine.disease_cause, biology.organism_classification, Virology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Antigen, Monoclonal, medicine, Immunology and Allergy, Chlamydia trachomatis, Chlamydia muridarum, 030215 immunology |
| الوصف: | Chlamydia is responsible for millions of new infections annually, and current efforts focus on understanding cellular immunity for targeted vaccine development. The Chlamydia-specific CD4 T cell response is characterized by the production of IFN-γ, and polyfunctional Th1 responses are associated with enhanced protection. A major limitation in studying these responses is the paucity of tools available for detection, quantification, and characterization of polyfunctional Ag-specific T cells. We addressed this problem by developing a TCR-transgenic (Tg) mouse with CD4 T cells that respond to a common Ag in Chlamydia muridarum and Chlamydia trachomatis. Using an adoptive-transfer approach, we show that naive Tg CD4 T cells become activated, proliferate, migrate to the infected tissue, and acquire a polyfunctional Th1 phenotype in infected mice. Polyfunctional Tg Th1 effectors demonstrated enhanced IFN-γ production compared with polyclonal cells, protected immune-deficient mice against lethality, mediated bacterial clearance, and orchestrated an anamnestic response. Adoptive transfer of Chlamydia-specific CD4 TCR-Tg T cells with polyfunctional capacity offers a powerful approach for analysis of protective effector and memory responses against chlamydial infection and demonstrates that an effective monoclonal CD4 T cell response may successfully guide subunit vaccination strategies. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ec198a1b4e48bba3c547472ed31dff85Test https://doi.org/10.4049/jimmunol.1700914Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ec198a1b4e48bba3c547472ed31dff85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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