Analysis of age-dependent DNA methylation changes in plucked hair samples using massive parallel sequencing
| العنوان: | Analysis of age-dependent DNA methylation changes in plucked hair samples using massive parallel sequencing |
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| المؤلفون: | Ulrike Schmidt, Sabine Lutz-Bonengel, Julia Winkelmann, Jana Naue |
| المصدر: | Rechtsmedizin. 31:226-233 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, Massive parallel sequencing, integumentary system, Age dependent, Biology, Amplicon, Molecular biology, Pathology and Forensic Medicine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, chemistry, CpG site, DNA methylation, 030216 legal & forensic medicine, Epigenetics, Degraded dna, DNA, 030304 developmental biology |
| الوصف: | The analysis of age-dependent DNA methylation changes is a valuable tool in epigenetic research and forensic genetics. With some exceptions, most studies in the past concentrated on the analysis of blood, buccal, and saliva samples. Another important sample type in forensic investigations is hair, where age-dependent DNA methylation has not been investigated so far. In this pilot study a deeper look was taken at the possibilities and challenges of DNA methylation analysis in hair. The DNA methylation of selected age-dependent 5’-C-phosphate-G‑3’ (CpG) sites were characterized for their potential use as a biomarker for age prediction using plucked hair samples and massive parallel sequencing. Plucked hair roots of 49 individuals were included in the study. The DNA methylation of 31 hairs was successfully analyzed. The DNA methylation pattern of 10 loci, including ELOVL2, F5, KLF14, and TRIM59, was determined by amplicon-based massive parallel sequencing. Age-dependent changes were found for several markers. The results demonstrate the possible use of already established age-dependent markers but at the same time they have tissue/cell type-specific characteristics. Special challenges such as low amounts of DNA and degraded DNA as well as the possible heterogeneous cellular composition of plucked hair samples, have to be considered. |
| تدمد: | 1434-5196 0937-9819 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e02398472a3f408e40d2e2165a9c358cTest https://doi.org/10.1007/s00194-021-00487-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........e02398472a3f408e40d2e2165a9c358c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14345196 09379819 |
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