The Histopathological Characteristics Caused by Trionyx sinensis Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles (Pelodiscus sinensis)
| العنوان: | The Histopathological Characteristics Caused by Trionyx sinensis Hemorrhagic Syndrome Virus (TSHSV) and Comparative Proteomic Analysis of Liver Tissue in TSHSV-Infected Chinese Soft-Shelled Turtles (Pelodiscus sinensis) |
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| المؤلفون: | Zheng Cao, Lu Shujuan, Sunjian Lyv, Li Liu, Ye Xueping, Feng Lin |
| المصدر: | Intervirology. 60:19-27 |
| بيانات النشر: | S. Karger AG, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Liver injury, Kidney, Pathology, medicine.medical_specialty, biology, Molecular pathology, H&E stain, medicine.disease, Trionyx, biology.organism_classification, Virus, 03 medical and health sciences, 030104 developmental biology, Infectious Diseases, Real-time polymerase chain reaction, medicine.anatomical_structure, Virology, medicine, Pathogen |
| الوصف: | Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues of Pelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infected P. sinensis were analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues of P. sinensis. The typical clinical symptoms included hepatomegaly, fragility, spotty and severe congestion in liver tissue, and also obvious intestinal bleeding. The histopathological studies corroborated such lesions in the liver and kidney, etc. iTRAQ-based proteomic analysis revealed that there were 252 differentially expressed proteins in the liver tissue between healthy and infected P. sinensis, of which 118 proteins were upregulated and 134 proteins were downregulated. GO enrichment analysis and KEGG pathway analysis initially revealed the molecular mechanism of pathological changes in P. sinensis by TSHSV infection. The expression of some differentially expressed proteins was further confirmed by qRT-PCR. These results provided important information for the pathological diagnosis of TSHSV-caused disease, as well as the mechanism underlying TSHSV-caused disease. |
| تدمد: | 1423-0100 0300-5526 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::da78d028aeb4d0f3315200f63749712eTest https://doi.org/10.1159/000479795Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........da78d028aeb4d0f3315200f63749712e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230100 03005526 |
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