Cloning and characterization of two osmotin isoforms from Piper colubrinum
| العنوان: | Cloning and characterization of two osmotin isoforms from Piper colubrinum |
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| المؤلفون: | T. Mani, S. Manjula |
| المصدر: | Biologia plantarum. 54:377-380 |
| بيانات النشر: | Institute of Experimental Botany, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cloning, Gene isoform, Molecular breeding, Genetics, cDNA library, Complementary DNA, Point mutation, Coding region, Plant Science, Horticulture, Biology, Gene |
| الوصف: | In the present study, we report the cloning and sequence characterization of two isoforms of osmotin, an antifungal PR-5 gene homologue, from a salicylic acid-induced subtracted cDNA library earlier generated in Piper colubrinum. The larger form of the gene is 693 bp long, encoding a 21.5 kDa protein. The smaller form comprises a 543 bp long coding sequence which code for a protein of 16.4 kDa. A notable feature of the smaller form was a prominent internal deletion of 150 bp besides certain point mutations. Cloned isoforms of osmotin from resistant species could be candidates for molecular breeding for the improvement of black pepper as well as candidates for the study of structure based mechanism of antifungal activity attributed to PR-5 family. |
| تدمد: | 1573-8264 0006-3134 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::d9971e26cdf028013d71e11ddd17eb10Test https://doi.org/10.1007/s10535-010-0068-1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........d9971e26cdf028013d71e11ddd17eb10 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15738264 00063134 |
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