Determination of metabolites of phloretin in rats using UHPLC-LTQ-Orbitrap mass spectrometry
| العنوان: | Determination of metabolites of phloretin in rats using UHPLC-LTQ-Orbitrap mass spectrometry |
|---|---|
| المؤلفون: | Liang Hong Liu, Zu Fu Yao, Ping Jin, Wei Cai, Hong Pu, Fang Ping Wu |
| المصدر: | Tropical Journal of Pharmaceutical Research. 18:2167-2173 |
| بيانات النشر: | African Journals Online (AJOL), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Chromatography, Phloretin, Electrospray ionization, technology, industry, and agriculture, Pharmaceutical Science, Orbitrap, Mass spectrometry, High-performance liquid chromatography, law.invention, Hydroxylation, chemistry.chemical_compound, chemistry, law, Pharmacology (medical), Glucuronide, Drug metabolism |
| الوصف: | Purpose: To study the metabolites of phloretin in vivo using ultra-high performance liquid chromatography linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap). Methods: After administration of phloretin (50 mg/kg; oral route) to six rats, blood samples were taken from each animal. Each sample was then subjected to solid-phase extraction to prepare it for chromatographic/spectroscopic analysis. Finally, each sample was analyzed using UHPLC-LTQOrbitrap with a negative-mode electrospray ionization source. Results: Based on mass measurements, chromatographic retention times, and MS2 fragmentation ions, we detected and identified phloretin and 16 metabolites of the drug in vivo in rats. Metabolic reactions of phloretin included glucosylation and glucuronide conjugation, diglucuronide conjugation, glucosylation and sulfate conjugation, sulfate conjugation, glucuronide conjugation, and glucosylation and hydroxylation. Conclusion: The findings provide a better understanding of phloretin metabolism and metabolites, and new information about their effective forms, pharmacological actions, metabolic fate, and toxic actions in vivo. |
| تدمد: | 1596-9827 1596-5996 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::d5070df0a1b8a8cacbe3ad15775a1766Test https://doi.org/10.4314/tjpr.v18i10.24Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........d5070df0a1b8a8cacbe3ad15775a1766 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
|---|