Propofol Downregulates lncRNA MALAT1 to Alleviate Cerebral Ischemia–Reperfusion Injury
| العنوان: | Propofol Downregulates lncRNA MALAT1 to Alleviate Cerebral Ischemia–Reperfusion Injury |
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| المؤلفون: | Junyang Chen, Shuang Cheng, Yubo Hu, Cong Ye |
| المصدر: | Inflammation. 44:2580-2591 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, medicine.diagnostic_test, Chemistry, Immunology, Ischemia, Pharmacology, medicine.disease, chemistry.chemical_compound, Western blot, In vivo, Apoptosis, Lactate dehydrogenase, biology.protein, medicine, TLR4, Immunology and Allergy, Creatine kinase, Reperfusion injury |
| الوصف: | Propofol (PPF) is reported to play a protective role in ischemia/reperfusion (I/R) injury, including cerebral ischemia-reperfusion injury (CIRI). This study aims to investigate the mechanism by which PPF ameliorates CIRI. Kunming mice were used to establish the middle cerebral artery occlusion (MCAO)/reperfusion mouse model in vivo. PPF pre-treatment was performed before CIRI. Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) levels were detected to evaluate the tissue injury. PC12 cells were exposed to hypoxia/reoxygenation (H/R) to construct the in vitro CIRI model, and PC12 cells were pre-treated with PPF before H/R. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression of lncRNA MALAT1 and miR-182-5p. Flow cytometry was used to detect the apoptosis of PC12 cells. Bioinformatics analysis, qRT-PCR, dual-luciferase reporter gene experiments, and RNA immunoprecipitation (RIP) experiments were performed to predict and validate the targeting relationship between MALAT1 and miR-182-5p. Western blot was used to detect Toll-like receptor 4 (TLR4) expression at protein level. PPF pre-treatment remarkably inhibited LDH and CPK levels in the serum of the mice with CIRI, and reduced the apoptosis of PC12 cells exposed to H/R. Besides, PPF pre-treatment markedly suppressed MALAT1 expression in both in vivo and in vitro models and upregulated miR-182-5p expression. MiR-182-5p was validated to be a downstream target gene of MALAT1, and MALAT1 could increase the expression of TLR4 by suppressing miR-182-5p. The effects of PPF on the injury of the mice brain and PC12 cells were partly counteracted by the restoration of MALAT1. PPF protects the brain against I/R-induced injury by regulating MALAT1/miR-182-5p/TLR4 axis. |
| تدمد: | 1573-2576 0360-3997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::d1831caf72907a23d961877668045ae2Test https://doi.org/10.1007/s10753-021-01525-9Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........d1831caf72907a23d961877668045ae2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15732576 03603997 |
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