The Glycosylphosphatidylinositol Anchor Regulates T Cell Antigen Receptor Induced IL-2 Production
| العنوان: | The Glycosylphosphatidylinositol Anchor Regulates T Cell Antigen Receptor Induced IL-2 Production |
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| المؤلفون: | Taroh Kinoshita, Dominik Filipp, Nathalie Vacaresse, Alessandra Ferzoco, Yutaka Amemiya, David Andrews, Michael Julius, Arun Seth |
| المصدر: | Open Journal of Immunology. 11:1-24 |
| بيانات النشر: | Scientific Research Publishing, Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Mutation, Messenger RNA, T-cell receptor, Stimulation, medicine.disease_cause, Transferase complex, Phenotype, Cell biology, carbohydrates (lipids), chemistry.chemical_compound, Biosynthesis, chemistry, medicine, lipids (amino acids, peptides, and proteins), Receptor |
| الوصف: | Differential contributions of the glycosylphosphatidylinositol (GPI)-anchor and GPI-anchored proteins (GPI-AP) to signalling remain poorly understood. Here we show that GPI-AP deficient murine clones produce on average 18 and 181-fold more IL-2 mRNA and protein, respectively, upon T cell receptor (TCR) stimulation, in a cell-intrinsic fashion. This phenotype is formally attributed to a mutation within the transferase complex that predicates the initial step in GPI-anchor biosynthesis. Conditional disruption of the transferase complex enabled the generation of primary GPI-AP deficient CD4+ T cells, which produce on average 10- and 23-fold more IL-2 mRNA and protein, respectively, upon TCR stimulation. Conditional disruption of the transamidase complex yields GPI-sufficient, GPI-AP deficient primary CD4+ T cells. TCR stimulation of these cells yields levels of IL-2 mRNA and protein ranging from 1 - 3 and 3-fold, respectively, of controls. These results provide the first evidence of a profound impact of GPI in the regulation of TCR signalling. |
| تدمد: | 2162-4526 2162-450X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::cc9034fad46ec78ccc6c0aeeb69610b4Test https://doi.org/10.4236/oji.2021.111001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........cc9034fad46ec78ccc6c0aeeb69610b4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21624526 2162450X |
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